重组人RASSF1蛋白(denatured) (ab137136)
Key features and details
- Expression system: Escherichia coli
- Purity: > 85% SDS-PAGE
- Tags: His-DDDDK tag N-Terminus
- Suitable for: SDS-PAGE
描述
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产品名称
重组人RASSF1蛋白(denatured) -
纯度
> 85 % SDS-PAGE. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMSGEPELIELRELA PAGRAGKGRTRLERANALRIARGTACNPTRQLVPGRGHRFQPAGPATHTW CDLCGDFIWGVVRKGLQCAHCKFTCHYRCRALVCLDCCGPRDLGWEPAVE RDTNVDEPVEWETPDLSQAEIEQKIKEYNAQINSNLFMSLNKDGSYTGFI KVQLKLVRPVSVPSSKKPPSLQDARRGPGRGTSVRRRTSFYLPKDAVKHL HVLSRTRAREVIEALLRKFLVVDDPRKFALFERAERHGQVYLRKLLDDEQ PLRLRLLAGPSDKALSFVLKENDSGEVNWDAFSMPELHNFLRILQREEEE HLRQILQKYSYCRQKIQEALHACPLG -
预测分子量
43 kDa including tags -
氨基酸
1 to 340 -
标签
His-DDDDK tag N-Terminus
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描述
重组人RASSF1蛋白
相关产品
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Related Products
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)
- Anti-RASSF1 antibody [OTI8E4] (ab119423)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab21536)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-6X His tag® antibody (ab9108)
技术指标
Our Abpromise guarantee covers the use of ab137136 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 2.4% Urea, 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine)
常规信息
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别名
- 123F2
- Cardiac specific ras association domain family 1 protein
- cardiac-specific ras association domain family 1 protein, 123F2
see all -
功能
Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK4 during Fas-induced apoptosis. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage. -
组织特异性
Isoform A and isoform C are ubiquitously expressed in all tissues tested, however isoform A is absent in many corresponding cancer cell lines. Isoform B is mainly expressed in hematopoietic cells. -
序列相似性
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Ras-associating domain.
Contains 1 SARAH domain. -
细胞定位
Nucleus. Predominantly nuclear and Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Nucleus. Localizes to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late telophase in cells. Colocalizes with MDM2 in the nucleus. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab137136 尚未被引用在任何文献中。