重组人PKN3蛋白(ab125615)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
描述
-
产品名称
重组人PKN3蛋白 -
生物活性
The specific activity of ab125615 was determined to be 3 nmol/mg/min.
-
纯度
> 75 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
-
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
-
种属
Human -
预测分子量
130 kDa including tags -
氨基酸
1 to 889 -
标签
GST tag N-Terminus
-
技术指标
Our Abpromise guarantee covers the use of ab125615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
Western blot
Functional Studies
SDS-PAGE
-
形式
Liquid -
补充说明
ab204879 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity
This product was previously labelled as PKN beta
-
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
-
别名
- pkn3
- PKN3_HUMAN
- PKNBETA
see all -
功能
Contributes to invasiveness in malignant prostate cancer. -
组织特异性
Expressed in prostate tumors and various cancer cell lines. Not expressed in adult tissues. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 3 REM (Hr1) repeats. -
结构域
The C1 domain does not bind the diacylglycerol (DAG). -
翻译后修饰
Autophosphorylated. -
细胞定位
Nucleus. Cytoplasm > perinuclear region. Nuclear and perinuclear Golgi region. - Information by UniProt
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab125615 尚未被引用在任何文献中。