重组人ITK/EMT蛋白(ab60300)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
描述
-
产品名称
重组人ITK/EMT蛋白
参阅全部 ITK/EMT 蛋白酶 -
生物活性
Specific activity: 58 nmol/min/mg.
-
纯度
> 90 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
-
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
-
种属
Human -
预测分子量
53 kDa -
氨基酸
352 to 620 -
标签
GST tag N-Terminus -
额外的序列信息
Recombinant fragment, corresponding to amino acids 352-end of Human ITK.
-
相关产品
-
Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab60300 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
Functional Studies
SDS-PAGE
-
形式
Liquid -
补充说明
ab43614 (Human Myelin Basic Protein full length protein) can be utilized as a substrate for assessing Kinase activity
Previously labelled as ITK.
-
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
-
别名
- EMT
- Homolog of mouse T cell itk/tsk
- IL 2 inducible T cell kinase
see all -
功能
Plays a role in T-cell proliferation and differentiation. -
组织特异性
T-cell lines and natural killer cell lines. -
疾病相关
Defects in ITK are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1) [MIM:613011]. LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk for lymphoma. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Contains 1 Btk-type zinc finger.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
细胞定位
Cell membrane. Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells. - Information by UniProt
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab60300 尚未被引用在任何文献中。