重组人IGF2蛋白(ab155617)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: Fc tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Achieve higher consistency and quality standards with a premium grade bioactive protein
- High batch-to-batch consistency
- Optimal bioactivity
- Guaranteed identical to human native proteins
- >95% purity
- Ultra-low endotoxin levels: <0.005 Eu/µg
- Carrier and tag free
描述
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产品名称
重组人IGF2蛋白
参阅全部 IGF2 蛋白酶 -
生物活性
Measured in a serum-free cell proliferation assay using MCF7 Human breast cancer cells.
The ED50 for this effect is typically 2-10 ng/ml. -
纯度
> 95 % SDS-PAGE.
Lyophilized from 0.22µm filtered solution -
内毒素水平
< 1.000 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
AYRPSETLCGGELVDTLQFVCGDRGFYFSRPASRVSRRSRGIVEECCFRS CDLALLETYCATPAKSE -
预测分子量
34 kDa -
分子量信息
The protein migrates as 35-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. -
氨基酸
25 to 91 -
标签
Fc tag N-Terminus -
额外的序列信息
This protein carries a human IgG1 Fc tag at the N-terminus.
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技术指标
Our Abpromise guarantee covers the use of ab155617 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 5% Trehalose, 0.75% Glycine, 0.605% TrisThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute with sterile deionized water to a concentration of 1 mg/ml.
常规信息
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别名
- C11orf43
- IGF 2
- IGF II
see all -
功能
The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3. -
疾病相关
Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations. -
序列相似性
Belongs to the insulin family. -
翻译后修饰
O-glycosylated with a core 1 or possibly core 8 glycan. -
细胞定位
Secreted. - Information by UniProt
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab155617 尚未被引用在任何文献中。