重组人IGF1蛋白(Active) (ab270062)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: < 0.005 Eu/µg
- Active: Yes
- Suitable for: Cell Culture, SDS-PAGE, HPLC, MS, Functional Studies
描述
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产品名称
重组人IGF1蛋白(Active)
参阅全部 IGF1 蛋白酶 -
生物活性
Fully biologicaly active as determined by dose-depended proliferation of MCF-7 cells.
ED50 is ≤ 0.9 ng/mL, corresponding to a specific activity of 1.11 x 106 units/mg.
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纯度
>= 95 % SDS-PAGE. -
内毒素水平
< 0.005 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
Yes -
无载体
是 -
性质
Recombinant -
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种属
Human -
序列
GP ETLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSA -
预测分子量
8 kDa -
分子量信息
M - 2.84 Da (Calculated mass 7711.84 Da) GP ETLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSA -
氨基酸
49 to 118 -
额外的序列信息
N-terminal glycine Full-length mature chain lacking the signal peptide and both pro-peptides.
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技术指标
Our Abpromise guarantee covers the use of ab270062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Cell Culture
SDS-PAGE
HPLC
Mass Spectrometry
Functional Studies
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形式
Lyophilized -
补充说明
This protein is filter sterilized prior to aliquoting and lyophilization. All aliquoting and lyophilization steps are performed in a sterile environment
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at Room Temperature. Store at Room Temperature.
pH: 6.00
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% Trehalose
Buffer lyophilized from.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
常规信息
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别名
- IBP1
- IGF I
- IGF IA
see all -
功能
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. -
疾病相关
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. -
序列相似性
Belongs to the insulin family. -
细胞定位
Secreted. - Information by UniProt
图片
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Fully biologicaly active as determined by dose-depended proliferation of MCF-7 cells. The ED50 is ≤ 0.9 ng/mL, corresponding to a specific activity of 1.11 x 106 units/mg.
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SDS-PAGE analysis of ab270062.
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Purity ≥95%
The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 µm). 5 µL of purified protein was injected and the gradient run from 80 % water:TFA (99.9:0.1 v/v) and 20 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) to 20 % water:TFA (99.9:0.1 v/v) and 80 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50 °C.
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M - 2.84 Da (Calculated mass 7711.84 Da)
The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 µm, Thermo Scientific). 5 µL of purified protein was injected and the gradient run from 85 % water:FA (99.9:0.1 v/v) and 15 % acetonitrile:FA (90:9.9:0.1 v/v/v) to 55 % water:FA (99.9:0.1 v/v) and 45 % acetonitrile:FA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60 °C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab270062 被引用在 5 文献中.
- Xu P et al. Hypoxia-mediated YTHDF2 overexpression promotes lung squamous cell carcinoma progression by activation of the mTOR/AKT axis. Cancer Cell Int 22:13 (2022). PubMed: 34996459
- Shokrollahi B et al. Apelin and Apelin Receptor in Follicular Granulosa Cells of Buffalo Ovaries: Expression and Regulation of Steroidogenesis. Front Endocrinol (Lausanne) 13:844360 (2022). PubMed: 35355567
- Bao YY et al. Effect of Glut-1 and HIF-1α double knockout by CRISPR/CAS9 on radiosensitivity in laryngeal carcinoma via the PI3K/Akt/mTOR pathway. J Cell Mol Med 26:2881-2894 (2022). PubMed: 35415942
- Xu K et al. IGF1 receptor-targeted black TiO2 nanoprobes for MRI-guided synergetic photothermal-chemotherapy in drug resistant pancreatic tumor. J Nanobiotechnology 20:315 (2022). PubMed: 35794573
- Judge RA et al. Structure of the PAPP-ABP5 complex reveals mechanism of substrate recognition. Nat Commun 13:5500 (2022). PubMed: 36127359