重组人HGF蛋白(Active) (ab632)

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Key features and details

  • Expression system: BTI-TN-5B1-4 cells
  • Purity: >= 98% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Active: Yes
  • Suitable for: HPLC, Functional Studies, SDS-PAGE

描述

  • 产品名称

    重组人HGF蛋白(Active)
    参阅全部 HGF 蛋白酶

  • 生物活性

    The ED50, as determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells, was found to be in the range of 20.0-40.0 ng/ml. 

  • 纯度

    >= 98 % SDS-PAGE.
    >=98% HPLC analyses. Sterile filtered.

  • 内毒素水平

    < 1.000 Eu/µg

  • 表达系统

    BTI-TN-5B1-4 cells

  • Accession

    P14210

  • 蛋白长度

    Full length protein

  • 无动物成分

    No

  • 性质

    Recombinant

    • 种属

      Human

    • 序列

      QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF NSMSSGVKKE FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP WSSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG LMDHTESGKI CQRWDHQTPH RHKFLPERYP DKGFDDNYCR NPDGQPRPWC YTLDPHTRWE YCAIKTCADN TMNDTDVPLE TTECIQGQGE GYRGTVNTIW NGIPCQRWDS QYPHEHDMTP ENFKCKDLRE NYCRNPDGSE SPWCFTTDPN IRVGYCSQIP NCDMSHGQDC YRGNGKNYMG NLSQTRSGLT CSMWDKNMED LHRHIFWEPD ASKLNENYCR NPDDDAHGPW CYTGNPLIPW DYCPISRCEG DTTPTIVNLD HPVISCAKTK QLRVVNGIPT RTNIGWMVSL RYRNKHICGG SLIKESWVLT ARQCFPSRDL KDYEAWLGIH DVHGRGDEKC KQVLNVSQLV YGPEGSDLVL MKLARPAVLD DFVSTIDLPN YGCTIPEKTS CSVYGWGYTG LINYDGLLRV AHLYIMGNEK CSQHHRGKVT LNESEICAGA EKIGSGPCEG DYGGPLVCEQ HKMRMVLGVI VPGRGCAIPN RPGIFVRVAY YAKWIHKIIL TYKVPQS

    • 预测分子量

      80 kDa

    • 氨基酸

      32 to 728

    • 额外的序列信息

      α-chain and β-chain, held together by a single disulfide bond, resulting in formation of a biologically active heterodimer. Full length mature protein, without the signal peptide.

相关产品

    技术指标

    Our Abpromise guarantee covers the use of ab632 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • 应用

      HPLC

      Functional Studies

      SDS-PAGE

    • 形式

      Lyophilized

    • 补充说明

      Expression System = Baculovirus infected High-5 cells.

    • Concentration information loading...

    制备和贮存

    • 稳定性和存储

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      Constituents: 1.16% Sodium chloride, 0.12% Tris, 1.74% DL-Arginine

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • 复溶
      Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.

    常规信息

    • 别名

      • DFNB39
      • F TCF
      • Fibroblast derived tumor cytotoxic factor
      • Hepatocyte growth factor
      • Hepatocyte growth factor (hepapoietin A; scatter factor)
      • Hepatocyte growth factor beta chain
      • Hepatocyte growth factor precursor
      • Hepatopoietin A
      • Hepatopoietin-A
      • Hgf
      • HGF_HUMAN
      • HGFB
      • HPTA
      • Lung fibroblast derived mitogen
      • OTTHUMP00000161349
      • OTTHUMP00000206710
      • OTTHUMP00000206711
      • OTTHUMP00000206712
      • OTTHUMP00000206713
      • OTTHUMP00000206730
      • Scatter factor
      • SF
      see all

    • 功能

      Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.

    • 疾病相关

      Deafness, autosomal recessive, 39

    • 序列相似性

      Belongs to the peptidase S1 family. Plasminogen subfamily.
      Contains 4 kringle domains.
      Contains 1 PAN domain.
      Contains 1 peptidase S1 domain.
    • Information by UniProt

    图片

    • Sandwich ELISA - Recombinant human HGF protein (ab632)
      Sandwich ELISA - Recombinant human HGF protein (ab632)
      Standard curve for HGF (Analyte: ab632); dilution range 1pg/ml to 1µg/ml using Capture Antibody ab10829 at 5µg/ml and Detector Antibody ab24865 at 0.5µg/ml.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

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    客户评价及客户问答

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    Question

    Is the protein HGF ab632 and ab4261 supplied as the active form?

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    Answer

    I can confirm the product ab632 human HGF is supplied as the active (mature form). Please contact us again if you need further information,

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