重组人HGF蛋白(Active) (ab259401)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, HPLC, MS, Functional Studies, Cell Culture
描述
-
产品名称
重组人HGF蛋白(Active)
参阅全部 HGF 蛋白酶 -
生物活性
Fully biologically active determined by the dose dependent induce IL-11 secretion by Saos-2 human osteosarcoma cells.
ED50 is ≤4.9 ng/ml, corresponding to a specific activity of 0.20 x 106 units/mg.
-
纯度
>= 95 % SDS-PAGE.
=95% by HPLC -
内毒素水平
<=0.005 Eu/µg -
表达系统
HEK 293 cells -
Accession
-
蛋白长度
Full length protein -
无动物成分
Yes -
无载体
是 -
性质
Recombinant -
-
种属
Human -
序列
QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF NSMSSGVKKE FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP WSSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG LMDHTESGKI CQRWDHQTPH RHKFLPERYP DKGFDDNYCR NPDGQPRPWC YTLDPHTRWE YCAIKTCADN TMNDTDVPLE TTECIQGQGE GYRGTVNTIW NGIPCQRWDS QYPHEHDMTP ENFKCKDLRE NYCRNPDGSE SPWCFTTDPN IRVGYCSQIP NCDMSHGQDC YRGNGKNYMG NLSQTRSGLT CSMWDKNMED LHRHIFWEPD ASKLNENYCR NPDDDAHGPW CYTGNPLIPW DYCPISRCEG DTTPTIVNLD HPVISCAKTK QLRVVNGIPT RTNIGWMVSL RYRNKHICGG SLIKESWVLT ARQCFPSRDL KDYEAWLGIH DVHGRGDEKC KQVLNVSQLV YGPEGSDLVL MKLARPAVLD DFVSTIDLPN YGCTIPEKTS CSVYGWGYTG LINYDGLLRV AHLYIMGNEK CSQHHRGKVT LNESEICAGA EKIGSGPCEG DYGGPLVCEQ HKMRMVLGVI VPGRGCAIPN RPGIFVRVAY YAKWIHKIIL TYKVPQS -
预测分子量
80 kDa -
实际分子量
80 kDa -
分子量信息
Predicted mass is 79720.38 Da(+/- 10 Da by ESI TOF). Observed mass is 79724.93 -
氨基酸
32 to 728 -
额外的序列信息
N-terminal glycine tag.
-
技术指标
Our Abpromise guarantee covers the use of ab259401 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
SDS-PAGE
HPLC
Mass Spectrometry
Functional Studies
Cell Culture
-
形式
Lyophilized -
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped at Room Temperature. Store at Room Temperature.
pH: 6.00
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
-
复溶Reconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
常规信息
-
别名
- DFNB39
- F TCF
- Fibroblast derived tumor cytotoxic factor
see all -
功能
Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. -
疾病相关
Deafness, autosomal recessive, 39 -
序列相似性
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 4 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain. - Information by UniProt
图片
-
Fully biologically active determined by the dose dependent induce IL-11 secretion by Saos‑2 human osteosarcoma cells.
ED50 is ≤4.9 ng/ml, corresponding to a specific activity of 0.20 x 106 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot: GR3369519-1
-
SDS-PAGE analysis of ab259401.
Additional lower MW bands are result of protein degradation during the production process and do not affect protein function.
-
Mass determination by ESI TOF.
Predicted mass is 79720.38 Da (+/- 10 Da by ESI TOF). Observed mass is 79724.93.
-
HPLC analysis of ab259401.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab259401 尚未被引用在任何文献中。