重组人HBEGF/DTR蛋白(Animal Free) (ab205523)
Key features and details
- Expression system: Escherichia coli
- Purity: > 97% SDS-PAGE
- Endotoxin level: < 0.050 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人HBEGF/DTR蛋白(Animal Free)
参阅全部 HBEGF/DTR 蛋白酶 -
生物活性
The activity is determined by the ability to induce proliferation of 3T3 cells and is typically less than 0.2 ng/mL. This corresponds to an expected specific activity of 5 x 106 units/mg.
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纯度
> 97 % SDS-PAGE. -
内毒素水平
< 0.050 Eu/µg -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
Yes -
性质
Recombinant -
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种属
Human -
序列
DLQEADLDLLRVTLSSKPQALATPNKEEHGKRKKKGKGLGKKRDPCLRKY KDFCIHGECKYVKELRAPSCICHPGYHGERCHGLSL -
预测分子量
10 kDa -
氨基酸
63 to 148 -
额外的序列信息
This is the full length protein without the signal peptide and propeptide.
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技术指标
Our Abpromise guarantee covers the use of ab205523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Lyophilized -
补充说明
This product was previously labelled as HB EGF
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon reconsitution add a carrier protein (0.1% BSA). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: 0.16% Sodium phosphate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute with sterile water at 0.1 mg/mL. Centrifuge vial before opening. Suspend the product by gently pipetting the sterile water down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.
常规信息
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别名
- Diphtheria toxin receptor
- diphtheria toxin receptor (heparin-binding EGF-like growth factor)
- diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor)
see all -
功能
May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor. -
序列相似性
Contains 1 EGF-like domain. -
翻译后修饰
Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937). -
细胞定位
Cell membrane and Secreted > extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab205523 被引用在 1 文献中.
- Luo P et al. s-HBEGF/SIRT1 circuit-dictated crosstalk between vascular endothelial cells and keratinocytes mediates sorafenib-induced hand-foot skin reaction that can be reversed by nicotinamide. Cell Res 30:779-793 (2020). PubMed: 32296111