概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 序列
      MGSSHHHHHH SSGLVPRGSH MADNFSLHDA LSGSGNPNPQ GWPGAWGNQP AGAGGYPGAS YPGAYPGQAP PGAYPGQAPP GAYPGAPGAY PGAPAPGVYP GPPSGPGAYP SSGQPSATGA YPATGPYGAP AGPLIVPYNL PLPGGVVPRM LITILGTVKP NANRIALDFQ RGNDVAFHFN PRFNENNRRV IVCNTKLDNN WGREERQSVF PFESGKPFKI QVLVEPDHFK VAVNDAHLLQ YNHRVKKLNE ISKLGISGDI DLTSASYTMI
    • 分子量
      28 kDa
    • 氨基酸
      1 to 250
    • 标签
      His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab89487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性

    The ED50 for this effect is ≤ 2.5 ug/ml. Measured by its ability to agglutinate human red blood cells.

  • 应用

    SDS-PAGE

  • 纯度
    > 95 % SDS-PAGE.
    Purified by using conventional chromatography techniques
  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.0154% DTT, 0.316% Tris HCl, 10% Glycerol, 0.58% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • 35 kDa lectin
    • Carbohydrate binding protein 35
    • Carbohydrate-binding protein 35
    • CBP 35
    • CBP35
    • Gal-3
    • GAL3
    • Galactose-specific lectin 3
    • Galactoside-binding protein
    • GALBP
    • Galectin 3 internal gene,included
    • Galectin-3
    • Galectin3
    • GALIG
    • GBP
    • IgE binding protein
    • IgE-binding protein
    • L 31
    • L 34
    • L-31
    • L-34 galactoside-binding lectin
    • L31
    • Laminin-binding protein
    • Lectin L-29
    • Lectin, galactose binding, soluble 3
    • LEG3_HUMAN
    • LGALS2
    • LGALS3
    • MAC 2 antigen
    • Mac-2
    • Mac-2 antigen
    • MAC2
    • Macrophage galactose-specific lectin
    • MGC105387
    see all
  • 功能
    Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
  • 组织特异性
    A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
  • 序列相似性
    Contains 1 galectin domain.
  • 细胞定位
    Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.
  • Information by UniProt

图片

  • 15% SDS-PAGE showing ab89487 at approximately 28.3kDa (3µg).

文献

This product has been referenced in:
  • Jiang K  et al. Galectin-3 regulates desmoglein-2 and intestinal epithelial intercellular adhesion. J Biol Chem 289:10510-7 (2014). Read more (PubMed: 24567334) »
See 1 Publication for this product

客户评价及客户问答

1-9 of 9 Q&A

Question
Answer

Thank you for contacting us. The concentration for ab89487 was determined by Bradford assay. I hope this helps, please let me know if you need any additional information or assistance.

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Answer

Thank you for your response. We are expecting the new stock any time in our US office and hopefully we can dispatch the item this week sometimes. Thank you for your understanding and patien

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Question
Answer

Thank you for your patience. This is to let you know that I have placed a new order for you - for one vial of ab89487 as a free of charge replacement and the new order number is 978590. Currently, we are waiting for the new stock and as soon as we get it we will dispatch your new order. One more note is that we have changed the storage information for this product on the on-line datasheet and as it states: " The product is shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles." I hope the new vial will work as it is expected, and please do let me know how you are getting on with this product.        

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Answer

Thank you for getting back to me. I completely understand your frustration and try to do my best to solve this problem as soon as possible. I would like to update you on this issue: The lot number is an identification number for the synthesis/purification. The product can be placed to our different stocks in different times and the batch numbers (GR 46331-3, GR 46331-2) represent when the actual aliquots were placed into our freezers. Since the lot you received was identical, there was no difference in purification in between the vials. I have asked my colleagues to get new vials from the stock as soon as possible. Thank you for your patience and understanding in this

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Question
Answer

Thank you for your response. We can provide new vial from the same lot. After reviewing and discussing this issue carefully with the originator, I would like to clarify that both Abcam  batch numbers GR 46331-3 (two free of charge replacement vials No:959627) and GR 46331-2 (No:972126) correspond to a unique lot (lot# 0942901). Furthermore, it was indeed the same lot which was shipped to you previously on two separate orders (No: 938829 and 898042). The originator didn’t produce a new lot at the time because after re-testing it, they found that the case was most likely isolated (the protein might have been damaged somewhere along the way). Just to confirm that we still have one vial from exactly the same lot in our US stock. I look forward to hearing from you soon.    

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Answer

Thank you for your enquiry and for getting back to me. I am very pleased to hear that that the two replacement vials from the very new batch worked fine. Just to clarify the new vials were specifically prepared for you and the new batch is still not ready to release. We have not received any similar complaints about the old batch so we have still kept it in stock in our different offices (UK/US/JP/HK). Since you are based in the US and myself work in the UK (Cambridge) office, in future it would be very much appreciated if you could specify which batch you would prefer to get when placing a new order. Otherwise my colleagues in the US Customer Service/Goods Out Department would not known if there was any particular issue with certain batch. Currently, we still have only one vial from the old batch and I am liaising with the Lab to get more vials from the new purification. I will get in touch with you as soon as I can. Thank you for your

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Answer

Thank you for your recent message. Could you please confirm if the replacement vials which were arranged on Order NO: 959627 worked as expected or not? This information would be very important for us and for our Lab. Did you purchase the new vial (GR 46331-3) after or before the new free of charge replacements were shipped to you? Have you requested a different batch while placing the new order? I look forward to hearing from you soon and hope to solve this as soon as possible.  

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Answer

Thank you for getting back to me. We will very shortly send you 2 vials from our new batch as free of charge replacements. We sincerely apologize for any inconvenience caused and thank you for bringing this matter to our attention. Please do let us know how you are getting on with this product. Good luck with your research!

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Answer

Thank you for providing some further details. I have contacted you on the 27th September but still not heard from you. To see whether it was an isolated case, we re-verified our previous tests and tested other vials from the same lot. We believe it was indeed an isolated case. We can certainly replace both vials or arrange a credit note or a full refund for you. Could you please get back to me and let me know how you wish to proceed. I apologize for any inconvenience caused and thank you for bringing this matter to our attention. I look forward to hearing from you soon.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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