重组人Galectin 3蛋白(Active) (ab89487)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人Galectin 3蛋白(Active)
参阅全部 Galectin 3 蛋白酶 -
生物活性
The ED50 for this effect is ≤ 15 ug/ml.
Measured by its ability to agglutinate human red blood cells.
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纯度
> 95 % SDS-PAGE.
Purified by using conventional chromatography techniques -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MADNFSLHDA LSGSGNPNPQ GWPGAWGNQP AGAGGYPGAS YPGAYPGQAP PGAYPGQAPP GAYPGAPGAY PGAPAPGVYP GPPSGPGAYP SSGQPSATGA YPATGPYGAP AGPLIVPYNL PLPGGVVPRM LITILGTVKP NANRIALDFQ RGNDVAFHFN PRFNENNRRV IVCNTKLDNN WGREERQSVF PFESGKPFKI QVLVEPDHFK VAVNDAHLLQ YNHRVKKLNE ISKLGISGDI DLTSASYTMI -
预测分子量
28 kDa including tags -
氨基酸
1 to 250 -
标签
His tag N-Terminus -
额外的序列信息
(NCBI NP_002297) MW confirmed by MALDI-TOF.
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相关产品
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Related Products
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-Galectin 3 antibody [A3A12] (ab2785)
- Anti-Galectin 3 antibody (ab31707)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-Galectin 3 antibody (ab53082)
- Anti-Galectin 3 antibody [EP2775Y] (ab76245)
- Anti-Galectin 3 antibody [EPR2774] (ab76466)
- Biotin Anti-Galectin 3 antibody (ab84238)
- Anti-6X His tag® antibody (ab9108)
技术指标
Our Abpromise guarantee covers the use of ab89487 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.0154% DTT, 0.316% Tris HCl, 10% Glycerol, 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- 35 kDa lectin
- Carbohydrate binding protein 35
- Carbohydrate-binding protein 35
see all -
功能
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. -
组织特异性
A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. -
序列相似性
Contains 1 galectin domain. -
细胞定位
Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab89487 被引用在 3 文献中.
- Mathew MP et al. Nutrient-responsive O-GlcNAcylation dynamically modulates the secretion of glycan-binding protein galectin 3. J Biol Chem 298:101743 (2022). PubMed: 35183508
- Stasenko M et al. Targeting galectin-3 with a high-affinity antibody for inhibition of high-grade serous ovarian cancer and other MUC16/CA-125-expressing malignancies. Sci Rep 11:3718 (2021). PubMed: 33580170
- Jiang K et al. Galectin-3 regulates desmoglein-2 and intestinal epithelial intercellular adhesion. J Biol Chem 289:10510-7 (2014). PubMed: 24567334