重组人Flt3 / CD135蛋白(Fc Chimera Active) (ab83998)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人Flt3 / CD135蛋白(Fc Chimera Active)
参阅全部 Flt3 / CD135 蛋白酶 -
生物活性
Activity: The ED50 of ab83998 is typically 1.0 -2.0 ng/ml as measured by its ability to neutralize Flt3 mediated proliferation of the OCI/AML5 human leukemia cell line.
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纯度
> 95 % SDS-PAGE. -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
Theoretical Sequence: NQDLPVIKCVLINHKNNDSSVGKSSSYPMVSESPEDLGC ALRPQSSGTVYEAAAVEVDVSASITLQVL VDAPGNISCLWVFKHSSLN CQPHFDLQNRGVVSMVILKMTETQAGEYLLFIQSEATNYTILFTVSIRNT L LYTLRRPYFRKMENQDALVCISESVPEPIVEWVLCDSQGESCKEESP AVVKKEEKVLHELFGTDIRCC ARNELGRECTRLFTIDLNQTPQTTLPQ LFLKVGEPLWIRCKAVHVNHGFGLTWELENKALEEGNYFEM STYSTNR TMIRILFAFVSSVARNDTGYYTCSSSKHPSQSALVTIVEKGFINATNSSE DYEIDQYEEFCFS VRFKAYPQIRCTWTFSRKSFPCEQKGLDNGYSISK FCNHKHQPGEYIFHAENDDAQFTKMFTLNIRRK PQVLAEASASQASCF SDGYPLPSWTWKKCSDKSPNCTEEITEGVWNRKANRKVFGQWVSSSTLNM SEAIKGFLVKCCAYNSLGTSCETILLNSPGPFPFIQDRIPKVDKKVEP KSCDKTHTCPPCPAPELLGGPS VFLFPPKPKDTLMISRTPEVTCVVVD VSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVL TVLHQDW LNGKEYKCRVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQV SLTCLVKGFYP SDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLT VDKSRWQQGNVFSCSVMHEALHNHYTQKS LSLSPGK -
额外的序列信息
Fused with the Fc region of Human IgG1 at the C-terminus.
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技术指标
Our Abpromise guarantee covers the use of ab83998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 1% Human serum albumin, 10% Trehalose
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
常规信息
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别名
- CD 135
- CD135
- CD135 antigen
see all -
功能
Receptor for the FL cytokine. Has a tyrosine-protein kinase activity. -
组织特异性
Bone marrow cells. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 protein kinase domain. -
细胞定位
Membrane. - Information by UniProt
图片
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Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
The triangle indicates the theoretical MW and pI of the protein. -
1D SDS-PAGE of ab83998 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab83998
Lane 2: ab83998 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab83998 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
10 μg protein loaded per lane; Deep Purple™ stained. -
A sample of ab83998 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 μg protein loaded. Spot train indicates presence of multiple isoforms.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab83998 尚未被引用在任何文献中。