重组人FLRT1蛋白(ab151663)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, HPLC
描述
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产品名称
重组人FLRT1蛋白
参阅全部 FLRT1 蛋白酶 -
纯度
> 95 % SDS-PAGE. -
内毒素水平
< 1.000 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
IDSTTCPSVCRCDNGFIYCNDRGLTSIPADIPDDATTLYLQNNQINNAGI PQDLKTKVNVQVIYLYENDLDEFPINLPRSLRELHLQDNNVRTIARDSLA RIPLLEKLHLDDNSVSTVSIEEDAFADSKQLKLLFLSRNHLSSIPSGLPH TLEELRLDDNRISTIPLHAFKGLNSLRRLVLDGNLLANQRIADDTFSRLQ NLTELSLVRNSLAAPPLNLPSAHLQKLYLQDNAISHIPYNTLAKMRELER LDLSNNNLTTLPRGLFDDLGNLAQLLLRNNPWFCGCNLMWLRDWVKARAA VVNVRGLMCQGPEKVRGMAIKDITSEMDECFETGPQGGVANAAAKTTASN HASATTPQGSLFTLKAKRPGLRLPDSNIDYPMATGDGAKTLAIHVKALTA DSIRITWKATLPASSFRLSWLRLGHSPAVGSITETLVQGDKTEYLLTALE PKSTYIICMVTMETSNAYVADETPVCAKAETADSYGPTTTLNQEQNAGPM ASLP -
氨基酸
21 to 524 -
标签
His tag C-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab151663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
HPLC
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形式
Lyophilized -
补充说明
Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term. Avoid freeze / thaw cycle. Please see notes section.
pH: 7.20
Constituents: 99% Phosphate Buffer, 0.88% Sodium chloride -
复溶Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
常规信息
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别名
- Fibronectin-like domain containing leucine rich transmembrane protein 1
- Fibronectin-like domain-containing leucine-rich transmembrane protein 1
- FLRT1
see all -
功能
Plays a role in fibroblast growth factor-mediated signaling cascades that lead to the activation of MAP kinases. Promotes neurite outgrowth via FGFR1-mediated activation of downstream MAP kinases. Promotes an increase both in neurite number and in neurite length. May play a role in cell-cell adhesion and cell guidance via its interaction with ADGRL1/LPHN1 and ADGRL3. -
组织特异性
Expressed in kidney and brain. -
序列相似性
Contains 1 fibronectin type-III domain.
Contains 10 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain. -
翻译后修饰
N-glycosylated.
Phosphorylated in response to FGFR1 signaling, but is not a direct substrate of FGFR1 or SRC. A mutant where the Tyr phosphorylation sites have been replaced by Phe displays constitutive FGFR1-dependent activation of downstream MAP kinases.
Proteolytic cleavage in the juxtamembrane region gives rise to a soluble ectodomain. -
细胞定位
Cell membrane. Endoplasmic reticulum membrane. Cytoplasmic vesicle membrane. Cytoplasm, perinuclear region. Cell junction, focal adhesion. Secreted. Cell projection. Cell junction. In addition to its location at the cell membrane, colocalizes with FGFR1 in punctate perinuclear cytoplasmic vesicles. Detected along neurites and at contacts between neurite termini and other cells. Proteolytic cleavage gives rise to a shedded ectodomain. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab151663 尚未被引用在任何文献中。