重组人Fibrillarin蛋白(ab99443)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 85% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS
描述
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产品名称
重组人Fibrillarin蛋白 -
纯度
>= 85 % SDS-PAGE.
ab99443 is purified using conventional chromatography techniques. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MGSSHHHHHHSSGLVPRGSHRSMGKNVMVEPHRHEGVFICRGKEDALVTK NLVPGESVYGEKRVSISEGDDKIEYRAWNPFRSKLAAAILGGVDQIHIKP GAKVLYLGAASGTTVSHVSDIVGPDGLVYAVEFSHRSGRDLINLAKKRTN IIPVIEDARHPHKYRMLIAMVDVIFADVAQPDQTRIVALNAHTFLRNGGH FVISIKANCIDSTASAEAVFASEVKKMQQENMKPQEQLTLEPYERDHAVV VGVYRPPPKVKN -
预测分子量
29 kDa including tags -
氨基酸
83 to 321 -
标签
His tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab99443 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Mass Spectrometry
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质谱法
MALDI-TOF -
形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.4
Constituents: 0.0154% DTT, 0.316% Tris HCl, 0.0292% EDTA, 20% Glycerol (glycerin, glycerine), 1.16% Sodium chloride
常规信息
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别名
- 34 kD nucleolar scleroderma antigen
- 34 kDa nucleolar scleroderma antigen
- Fbl
see all -
功能
S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239). -
序列相似性
Belongs to the methyltransferase superfamily. Fibrillarin family. -
翻译后修饰
By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA). -
细胞定位
Nucleus, nucleolus. Fibrillar region of the nucleolus. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab99443 尚未被引用在任何文献中。