重组人ERK5蛋白(ab126913)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Active: Yes
- Suitable for: Functional Studies, WB, SDS-PAGE
描述
-
产品名称
重组人ERK5蛋白 -
生物活性
The specific activity of ab126913 was determined to be 11 nmol/min/mg
-
纯度
> 75 % Densitometry.
Purity was determined to be >75% by densitometry. Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
-
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
-
种属
Human -
预测分子量
132 kDa including tags -
氨基酸
1 to 816
-
相关产品
-
Related Products
-
Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab126913 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
Functional Studies
Western blot
SDS-PAGE
-
形式
Liquid -
补充说明
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
-
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
-
别名
- Big MAP kinase 1
- BMK 1
- BMK 1 kinase
see all -
功能
Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. -
组织特异性
Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
结构域
The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell.
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻译后修饰
Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme (By similarity). Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent. -
细胞定位
Cytoplasm. Nucleus. Translocates to the nucleus upon activation. - Information by UniProt
图片
-
The specific activity of ERK5 (ab126913) was determined to be 2.5 nmol/min/mg as per activity assay protocol and was equivalent to 12.7 nmol/min/mg as per radiometric assay
-
SDS PAGE analysis of ab126913
-
SDS PAGE analysis of ab126913
-
SDS-PAGE using ab126913
-
The specific activity of ab126913 was determined to be 11 nmol/min/mg
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab126913 被引用在 1 文献中.
- Liu W et al. Metabolic stress-induced cardiomyopathy is caused by mitochondrial dysfunction due to attenuated Erk5 signaling. Nat Commun 8:494 (2017). PubMed: 28887535