重组人COX1 / Cyclooxygenase 1蛋白(ab198643)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His-DDDDK tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人COX1 / Cyclooxygenase 1蛋白 -
生物活性
Mixed various concentrations of enzyme in Assay buffer (100 mM Tris-HCl pH 8, 3 µM EDTA) with 5 µM Hematin for 10 min
then added 100 µM Ampliflu Red and 200 μM arachidonic acid. Fluorescence (ex. 535 em. 590) was measured after 5
minutes at room temperature. Reaction volume was 100 μl. Application: Useful for the study of enzyme kinetics, screening inhibitors and selectivity profiling. -
纯度
>= 90 % SDS-PAGE.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MSRSLLLWFLLFLLLLPPLPVLLADPGAPTPVNPCCYYPCQHQGICVRFG LDRYQCDCTRTGYSGPNCTIPGLWTWLRNSLRPSPSFTHFLLTHGRWFWE FVNATFIREMLMRLVLTVRSNLIPSPPTYNSAHDYISWESFSNVSYYTRI LPSVPKDCPTPMGTKGKKQLPDAQLLARRFLLRRKFIPDPQGTNLMFAFF AQHFTHQFFKTSGKMGPGFTKALGHGVDLGHIYGDNLERQYQLRLFKDGK LKYQVLDGEMYPPSVEEAPVLMHYPRGIPPQSQMAVGQEVFGLLPGLMLY ATLWLREHNRVCDLLKAEHPTWGDEQLFQTTRLILIGETIKIVIEEYVQQ LSGYFLQLKFDPELLFGVQFQYRNRIAMEFNHLYHWHPLMPDSFKVGSQE YSYEQFLFNTSMLVDYGVEALVDAFSRQIAGRIGGGRNMDHHILHVAVDV IRESREMRLQPFNEYRKRFGMKPYTSFQELVGEKEMAAELEELYGDIDAL EFYPGLLLEKCHPNSIFGESMIEIGAPFSLKGLLGNPICSPEYWKPSTFG GEVGFNIVKTATLKKLVCLNTKTCPYVSFRVPDASQDDGPAVERPSTELD YKDDDDKHHHHHH -
预测分子量
71 kDa including tags -
氨基酸
1 to 599 -
标签
His-DDDDK tag C-Terminus -
额外的序列信息
NM_000962.
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相关产品
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Related Products
- Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)
- Anti-COX1 / Cyclooxygenase 1 antibody [EPR5867] (ab133319)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-6X His tag® antibody (ab9108)
技术指标
Our Abpromise guarantee covers the use of ab198643 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
补充说明
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine), 0.08% Triton X-100, 0.0067% Diethyldithiocarbamate
Contains 80 µg/ml DDDDK peptide.This product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- COX 1
- COX 3
- COX-1
see all -
功能
May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells. -
通路
Lipid metabolism; prostaglandin biosynthesis. -
序列相似性
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
细胞定位
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
图片
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4-20% SDS-PAGE analysis of 1.2 µg ab198643 with Coomassie staining.
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Mixed various concentrations of enzyme in Assay buffer (100 mM Tris-HCl pH 8, 3 µM EDTA) with 5 µM Hematin for 10 min then added 100 µM Ampliflu Red and 200 μM arachidonic acid. Fluorescence (ex. 535 em. 590) was measured after 5 minutes at room temperature. Reaction volume was 100 μl.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab198643 尚未被引用在任何文献中。