重组人CLSTN1蛋白(ab182805)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His-T7 tag N-Terminus
- Suitable for: SDS-PAGE
描述
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产品名称
重组人CLSTN1蛋白
参阅全部 CLSTN1 蛋白酶 -
纯度
> 90 % SDS-PAGE.
ab182805 is expressed in E.coli as inclusion bodies. The final product was refolded and chromatographically purified. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MASMTGGQQMGRGHHHHHHGNLYFQGGEFARVNKHKPWLEPTYHGIVTEN DNTVLLDPPLIALDKDAPLRFAESFEVTVTKEGEICGFKIHGQNVPFDAV VVDKSTGEGVIRSKEKLDCELQKDYSFTIQAYDCGKGPDGTNVKKSHKAT VHIQVNDVNEYAPVFKEKSYKATVIEGKQYDSILRVEAVDADCSPQFSQI CSYEIITPDVPFTVDKDGYIKNTEKLNYGKEHQYKLTVTAYDCGKKRATE DVLVKISIKPTCTPGWQGWNNRIEYEPGTGALAVFPNIHLETCDEPVASV QATVELETSHIGKGCDRDTYSEKSLHRLCGAAAGTAELLPSPSGSLNWTM GLPTDNGHDSDQVFEFNGTQAVRIPDGVVSVSPKEPFTISVWMRHGPFGR KKETILCSSDKTDMNRHHYSLYVHGCRLIFLFRQDPSEEKKYRPAEFHWK LNQVCDEEWHHYVLNVEFPSVTLYVDGTSHEPFSVTEDYPLHPSKIETQL VVGACWQEFSGVENDNETEPVTVASAGGDLHMTQFFRGNLAGLTLRSGKL ADKKVIDCLYTCKEGLDLQVLEDSGRGVQIQAHPSQLVLTLEGEDLGELD KAMQHISYLNSRQFPTPGIRRLKITSTIKCFNEATCISVPPVDGYVMVLQ PEEPKISLSGVHHFARAASEFESSEGVFLFPELRIISTITREVEPEGDGA EDPTVQESLVSEEIVHDLDTCEVTVEGEELNHEQESLEVDMARLQQKGIE VSSSELGMTFTGVDTMASYEEVLHLLRYRNWHARSLLDRKFKLICSELNG RYISNEFKVEVNVIHTANPMEHANHMAAQPQFVHPEHRSFVDLSGHNLAN PHPFAVVPST -
预测分子量
96 kDa including tags -
氨基酸
29 to 859 -
标签
His-T7 tag N-Terminus -
额外的序列信息
The extracellular domain of CLSTN1 constructed with codon optimization and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal. NP_055759.
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab182805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituent: 0.32% Tris HCl
Buffer also contains NaCl, KCl, EDTA, arginine, DTT and Glycerol.
常规信息
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别名
- Alc alpha
- Alc-alpha
- Alcadein alpha
see all -
功能
Induces KLC1 association with vesicles and functions as a cargo in axonal anterograde transport. Complex formation with APBA2 and APP, stabilizes APP metabolism and enhances APBA2-mediated suppression of beta-APP40 secretion, due to the retardation of intracellular APP maturation. In complex with APBA2 and C99, a C-terminal APP fragment, abolishes C99 interaction with PSEN1 and thus APP C99 cleavage by gamma-secretase, most probably through stabilization of the direct interaction between APBA2 and APP. The intracellular fragment AlcICD suppresses APBB1-dependent transactivation stimulated by APP C-terminal intracellular fragment (AICD), most probably by competing with AICD for APBB1-binding. May modulate calcium-mediated postsynaptic signals. -
组织特异性
Expressed in the brain and, a lower level, in the heart, skeletal muscle, kidney and placenta. Accumulates in dystrophic neurites around the amyloid core of Alzheimer disease senile plaques (at protein level). -
序列相似性
Contains 2 cadherin domains. -
结构域
The cytoplasmic domain is involved in interaction with APBA2, as well as the binding of synaptic Ca(2+). -
翻译后修饰
Proteolytically processed under normal cellular conditions. A primary zeta-cleavage generates a large extracellular (soluble) N-terminal domain (sAlc) and a short C-terminal transmembrane fragment (CTF1). A secondary cleavage catalyzed by presenilin gamma-secretase within the transmembrane domain releases the beta-Alc-alpha chain in the extracellular milieu and produces an intracellular fragment (AlcICD). This processing is strongly suppressed in the tripartite complex formed with APBA2 and APP, which seems to prevent the association with PSEN1. -
细胞定位
Endoplasmic reticulum membrane. Golgi apparatus membrane. Cell projection. Cell junction > synapse > postsynaptic cell membrane. Nucleus. Neurite tips. Localized in the postsynaptic membrane of both excitatory and inhibitory synapses (By similarity). The AlcICD fragment is translocated to the nucleus upon interaction with APBB1. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab182805 尚未被引用在任何文献中。