重组人Cdk4 + Cyclin D1蛋白(ab55695)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: WB, Functional Studies
描述
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产品名称
重组人Cdk4 + Cyclin D1蛋白 -
生物活性
Specific Activity is lot specific. Please contact our technical team to get lot specific information.
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纯度
> 75 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
标签
GST tag N-Terminus -
额外的序列信息
MW: Cdk4 = 57 kDa and CyclinD1 = 61 kDa.
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相关产品
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Related Products
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab55695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
Functional Studies
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形式
Liquid -
补充说明
14nmol/min/mg using protein substrate Rb (773-928).ab56270 (Human Rb protein fragment) can be utilized as a substrate for assessing Kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.00174% PMSF, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.31% GlutathioneThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- B cell CLL lymphoma 1
- BCL 1 oncogene
- BCL1
see all -
相关性
CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase. Phosphorylation at Thr-172 is necessary for enzymatic activity. CDK4 mutations are involved in tumor formation, with defects in CDK4 resulting in cutaneous malignant melanoma 3 (CMM3). Cyclin D1 is essential for the control of the cell cycle at the G1/S transition. It interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. -
细胞定位
Nuclear
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab55695 被引用在 6 文献中.
- McCann JL et al. The DNA deaminase APOBEC3B interacts with the cell-cycle protein CDK4 and disrupts CDK4-mediated nuclear import of Cyclin D1. J Biol Chem 294:12099-12111 (2019). PubMed: 31217276
- Wang Y et al. Design, synthesis and biological evaluation of pyrimidine derivatives as novel CDK2 inhibitors that induce apoptosis and cell cycle arrest in breast cancer cells. Bioorg Med Chem 26:3491-3501 (2018). PubMed: 29853338
- Wang H et al. The metabolic function of cyclin D3-CDK6 kinase in cancer cell survival. Nature 546:426-430 (2017). PubMed: 28607489
- Kondo E et al. Tumour lineage-homing cell-penetrating peptides as anticancer molecular delivery systems. Nat Commun 3:951 (2012). PubMed: 22805558
- Cho HS et al. RB1 methylation by SMYD2 enhances cell cycle progression through an increase of RB1 phosphorylation. Neoplasia 14:476-86 (2012). PubMed: 22787429
- Nakajima K et al. Coordinated regulation of differentiation and proliferation of embryonic cardiomyocytes by a jumonji (Jarid2)-cyclin D1 pathway. Development 138:1771-82 (2011). PubMed: 21447557