重组人Casein Kinase 1 delta/CSNK1D蛋白(ab103955)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% Densitometry
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, WB
描述
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产品名称
重组人Casein Kinase 1 delta/CSNK1D蛋白
参阅全部 Casein Kinase 1 delta/CSNK1D 蛋白酶 -
生物活性
The Specific activity of ab103955 was determined to be 82 nmol/min/mg.
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纯度
> 80 % Densitometry.
Purity was determined to be >80% by densitometry. Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
72 kDa including tags -
氨基酸
1 to 415
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab103955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
Western blot
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形式
Liquid -
补充说明
ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity
Previously labelled as Casein Kinase 1 delta.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- Casein Kinase 1 delta
- Casein kinase I delta isoform
- Casein kinase I isoform delta
see all -
功能
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm. -
组织特异性
Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. In blood, highly expressed in hemopoietic cells and mature granulocytes. Also found in monocytes and lymphocytes. -
疾病相关
Defects in CSNK1D are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms. -
序列相似性
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated on serine and threonine residues. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
图片
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The specific activity of Casein Kinase 1 delta/CSNK1D (ab103955) was determined to be 77 nmol/min/mg as per activity assay protocol
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SDS PAGE analysis of ab103955
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SDS PAGE analysis of ab103955
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The Specific activity of ab103955 was determined to be 82 nmol/min/mg.
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SDS-PAGE showing ab103955 at approximately 72kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab103955 被引用在 2 文献中.
- Ibrahim H et al. Phosphorylation of GAPVD1 Is Regulated by the PER Complex and Linked to GAPVD1 Degradation. Int J Mol Sci 22:N/A (2021). PubMed: 33917494
- Hanna-Addams S et al. CK1a, CK1d, and CK1e are necrosome components which phosphorylate serine 227 of human RIPK3 to activate necroptosis. Proc Natl Acad Sci U S A 117:1962-1970 (2020). PubMed: 31932442