Application
Western blot
Review text: Used us a positive control in western blot among ChIP samples.
Sample: Human Cell lysate - nuclear (colon)
Loading amount: 0.01 µg
Specification: colon
Gel Running Conditions: Reduced Denaturing (4-12%)
Blocking step: Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 4°C
Other product details
Dilution: 1/1000
Incubation time: 16 hour(s) and 0 minute(s) · Temperature: 4°C
Primary antibody (in addition to 'c-Myc protein (Human)')
Dilution: 1/1000
Secondary antibody
Name: Abcam antibody:(not specified yet)
Dilution: 1/1000
Detection
Detection method: ECL substrate
Exposure: 1 minute(s) and 0 second(s)
Bands: Specific: around 55 kDa
Negative control: BSA ChIPed cell lysate nuclear
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Aug 26 2011