重组HDAC3+NCOR2 complex蛋白(ab42631)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: Inhibition Assay
描述
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产品名称
重组HDAC3+NCOR2 complex蛋白 -
生物活性
Activity: 110 U/ug. One U =1 pmol/min, Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 30 uM Biomol substrate (Catalog number KI177), and 1 ng/ul HDAC3/NcoR2. Incubation condition: 30 min at 37ºC.
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纯度
> 90 % SDS-PAGE.
Affinity purified. -
表达系统
Baculovirus infected insect cells -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant
相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab42631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Inhibition Assay
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形式
Liquid -
补充说明
Co-expressed in a Baculovirus infected Sf9 cell expression system. -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.614% Glutathione, 0.79% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.8004% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- CTG26
- HD 3
- HD3
see all -
功能
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the histone deacetylase family. HD type 1 subfamily. -
翻译后修饰
Sumoylated in vitro. -
细胞定位
Nucleus. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab42631 被引用在 1 文献中.
- Demmerle J et al. The nuclear envelope protein emerin binds directly to histone deacetylase 3 (HDAC3) and activates HDAC3 activity. J Biol Chem : (2012). PubMed: 22570481