重组E. coli Multi Tag蛋白(ab36791)
Key features and details
- Expression system: Escherichia coli
- Suitable for: WB
描述
-
产品名称
重组E. coli Multi Tag蛋白 -
生物活性
This protein lysate contains the commonly used epitope tags: HA, c-myc, Glu-Glu, GST, 6-His, HSV, T7, V5, VSV, E-tag, S-tag, FLAG (TM). These proteins were fused in the following order, GST, T7, HSV, c-myc, VSV, Glu-Glu, V5, E-tag, DYKDDDDK, S-tag and HA, 6xHis. -
表达系统
Escherichia coli -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
-
种属
Escherichia coli -
预测分子量
45 kDa
-
-
描述
重组E. coli Multi Tag蛋白
技术指标
Our Abpromise guarantee covers the use of ab36791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
Western blot
-
形式
Liquid -
补充说明
This protein lysate contains the commonly used epitope tags: HA, c-myc, Glu-Glu, GST, 6-His, HSV, T7, V5, VSV, E-tag, S-tag, FLAG (TM). These proteins were fused in the following order, GST, T7, HSV, c-myc, VSV, Glu-Glu, V5, E-tag, DYKDDDDK, S-tag and HA, 6xHis.
This product is a bacterial lysate provided in1 x reduced SDS-PAGE loading buffer. It is effective as a positive control for epitope tag antibodies in immunoblotting.
For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µl. It is recommended that the reagent be titrated for optimal performance for each application.
-
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 6.80
Constituents: 0.76% Tris, 2% Sodium lauryl sulfate, 10% Glycerol, 0.01% Bromophenol blue
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab36791 被引用在 1 文献中.
- Trofka A et al. The Her7 node modulates the network topology of the zebrafish segmentation clock via sequestration of the Hes6 hub. Development 139:940-7 (2012). WB . PubMed: 22278920