重组食蟹猴LDL Receptor蛋白(Active) (ab220535)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组食蟹猴LDL Receptor蛋白(Active)
参阅全部 LDL Receptor 蛋白酶 -
生物活性
Measured by its binding ability in a functional ELISA. Immobilized ab220535 at 2 μg/ml (100 μl/well) can bind Biotinylated Human PCSK9, His Tag with a linear range of 0.007-0.256 μg/ml.
Immobilized Cynomolgus PCSK9, His Tag at 10 μg/ml (100 μl/well) can bind ab220535 with a linear range of 0.04-0.256 μg/ml.
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纯度
> 95 % SDS-PAGE. -
内毒素水平
< 1.000 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Cynomolgus monkey -
序列
AVGDRCERNEFQCEDGKCISYKWVCDGTAECQDGSDESQETCLSVTCKSG DFSCGGRVNRCIPQFWRCDGEVDCENGSDEQDCPPKTCSQDEFRCHDGKC IYRQFVCDSDRDCLDGSDEASCPVLTCGPASFQCNSSTCIPQLWACDNDP DCEDGSDEWPQHCQGLEVPKRDSSPCSAFEFHCRSGECIHSGWRCDGGPD CKDKSDEENCPVATCRPDEFQCSDGTCIHGSRQCDREYDCKDMSDEVGCI NVTLCEGPNKFKCHSGECISLDKVCNMARDCRDWSDEPIKECGTNECLDN NGGCSHICNDLKIGYECLCPDGFQLVAQRRCEDIDECQDPDTCSQLCVNL EGSYKCQCEEGFQLDPHTKACKAVGSIAYLIFTNRHEVRKMTLDRSEYTS LIPNLRNVVALDTEVASNRIYWSDLSQRMIYSTQLDRAHSVSSYDTVISR DLQAPDGLAVDWIHSNIYWTDSVLGTVSVADTKGVKRKTLFRENGSKPRA IVVDPVHGFMYWTDWGTPAKIKKGGLNGVDIYSLVTENIEWPNGITLDFP SGRLYWVDSKLHSISSIDVNGGNRKTVLEDKERLAHPFSLAIFEDKVFWT DIINEAIFSANRLTGSDINLLAENLLSPEDMVLFHNLTQPRGVNWCERTT LSNGGCQYLCLPAPQINPQSPKFTCTCPDGMLLAKDMRSCLTEAEAAVAT QETSTVRLMVSSKAVATQHTTTRPVPNTSQLPGATPGLTTAETVTMSHQA LGDVAGRGNEKKPKSVG -
预测分子量
87 kDa including tags -
氨基酸
22 to 788 -
标签
His tag C-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab220535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 95% PBS, 5% Trehalose
Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute with sterile deionized water to a concentration of 200 µg/ml.
常规信息
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别名
- FH
- FHC
- LDL R
see all -
功能
Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. -
疾病相关
Defects in LDLR are the cause of familial hypercholesterolemia (FH) [MIM:143890]; a common autosomal semi-dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis). -
序列相似性
Belongs to the LDLR family.
Contains 3 EGF-like domains.
Contains 7 LDL-receptor class A domains.
Contains 6 LDL-receptor class B repeats. -
翻译后修饰
N- and O-glycosylated.
Ubiquitinated by MYLIP leading to degradation. -
细胞定位
Cell membrane. Endomembrane system. Membrane > clathrin-coated pit. Found distributed from the plasma membrane to intracellular compartments. - Information by UniProt
图片
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SDS-PAGE analysis of ab220535 stained overnight with Coomassie Blue.
The protein migrates as 135-155 kDa under reducing conditions due to glycosylation.
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Immobilized ab220535 at 2 μg/ml (100 μl/well) can bind Biotinylated Human PCSK9, His Tag with a linear range of 0.007-0.256 μg/ml.
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Immobilized Cynomolgus PCSK9, His Tag at 10 μg/ml (100 μl/well) can bind ab220535 with a linear range of 0.04-0.256 μg/ml.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab220535 尚未被引用在任何文献中。