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After immunochemical staining, mounting media are used to adhere a coverslip to a tissue section or cell smear. Mounting media are important to protect the specimen and staining from physical damage. Mounting media also play an important role in improving the clarity and contrast of an image during microscopy.
There are two categories of mounting media, organic and aqueous (or hydrophobic and hydrophilic, respectively). Organic mounting media can only be used for enzymatic labels where the precipitate formed between the enzyme and the chromogen is not soluble in the organic solvents used during mounting of the tissue (eg diaminobenzidine, DAB).
Aqueous mounting media are generally suitable for all enzymatic label/chromogen combinations and fluorescent labels.
Product name | Type | Product code |
Limonene Mounting Medium | Organic | ab104141 |
Mounting Medium for IHC | Aqueous | ab64230 |
For fluorescent imaging, mounting media that preserve fluorescence in tissue and cytological smears, are used, enabling imaging of samples after prolonged storage.
Product name | Type | Product code |
Anti-Fade Mounting Medium | Aqueous | ab104135 |
Anti-Fade Mounting Medium With DAPI | Aqueous | ab104139 |
Anti-Fade Mounting Medium With PI | Aqueous | ab104129 |
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