CRISPR knock-out cell line publications

CRISPR-Cas9 engineered knock-out cell lines are helping scientists across the globe to break new boundaries within life science. From validating biotech targets to accelerating the clinical pipeline, CRISPR knock-out cell lines are providing the analytical power to ask new questions, drive new avenues of proteomic research and provide answers that are driving medicine forward.

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Here we have collated some of the most recent key CRISPR knock-out cell line publications, showing how these CRISPR knock-out cell lines are currently being used to push the bounds of biomedical research.

• RIPK2 Stabilizes c-Myc and is an actionable target for inhibiting prostate cancer metastasis

Yan Y, Zhou B, Qian C, et al. bioRxiv. (2020) doi: https://doi.org/10.1101/2020.05.14.096867

CRISPR knock-out HEK293T cells negative for RIPK2, MAP2K7, and PRKDC were used in this study to demonstrate the potential of RIPK2 as an actionable drug target for the personalized treatment of advanced prostate cancer, by preventing the activation of the MKK7/JNK/c-Myc phosphorylation pathway and promoting the degradation of c-Myc.

• Fenretinide induces a new form of dynamin-dependent cell death in pediatric sarcoma

Brack E, Wachtel M, Wolf A, et al. Cell Death Differ. (2020) 27(8):2500-2516

CRISPR knock-out cell lines negative for a large dynamin GTPase helped determine a new form of dynamin-dependent programmed cell death mediated through the production of reactive oxygen species by treatment with fenretinide, the drug used for treating childhood sarcomas.

• Loss of Caspase-8 function in combination with SMAC mimetic treatment sensitizes Head and Neck Squamous Carcinoma to radiation through induction of necroptosis

Uzunparmak B, Gao M, Lindemann A,  et al. bioRxiv (2020) https://doi.org/10.1101/2020.04.17.039040

A CRISPR knock-out murine oral squamous cell carcinoma cell line negative for Caspase-8 was used to promote the understanding of the effects of mutations in this gene in head and neck squamous cell carcinoma (HNSCC). CASP8 is shown to regulate necroptosis in HSNCC with the potential to therapeutically exploit this pathway.

• Neuropathy-causing mutations in HSPB1 impair autophagy by disturbing the formation of SQSTM1 p62 bodies

Haidar M, Asselbergh B, Adriaenssens E, et al. Autophagy. (2019) 15(6):1051-1068

Using HSPB1^-/- CRISPR knock-out HeLa cells this paper indicates that the molecular chaperone HSPB1 serves a regulatory role in autophagy via interaction with the SQSTM1/p62 autophagy receptor and show links with peripheral neuropathy in patient-derived motor neurons.

• PGAM5-MAVS interaction regulates TBK1/ IRF3 dependent antiviral responses

Yu Y, Zielinska M, Li W, Bernkopf DB, et al. Sci Rep. (2020) 20;10(1):8323

PGAM5^-/- CRISPR knock-out HeLa cells are used to demonstrate the role of PGAM5 in the regulation of IFNß in response to viral infection, as part of the innate immune system.