Anti-REA抗体(ab71970)
Key features and details
- Rabbit polyclonal to REA
- Suitable for: ICC/IF, IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-REA抗体
参阅全部 REA 一抗 -
描述
兔多克隆抗体to REA -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IP, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Orangutan, Elephant -
免疫原
Synthetic peptide corresponding to Human REA.
Database link: Q99623 -
阳性对照
- WB: HeLa, HEK-293T, NIH/3T3 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab71970于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 5 µg/ml.
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IP |
Use at an assay dependent concentration.
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WB |
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
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说明 |
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ICC/IF
Use a concentration of 5 µg/ml. |
IP
Use at an assay dependent concentration. |
WB
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). |
靶标
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功能
Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases (By similarity). Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging. -
序列相似性
Belongs to the prohibitin family. -
发展阶段
Levels of expression in fibroblasts decrease heterogeneously during cellular aging. -
细胞定位
Mitochondrion inner membrane. Cytoplasm. Nucleus. Also cytoplasmic and nuclear. - Information by UniProt
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数据库链接
- Entrez Gene: 515363 Cow
- Entrez Gene: 11331 Human
- Entrez Gene: 12034 Mouse
- Entrez Gene: 114766 Rat
- Omim: 610704 Human
- SwissProt: Q2HJ97 Cow
- SwissProt: Q99623 Human
- SwissProt: O35129 Mouse
see all -
别名
- B cell receptor associated protein BAP37 antibody
- B-cell receptor-associated protein BAP37 antibody
- BAP antibody
see all
图片
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All lanes : Anti-REA antibody (ab71970) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 37 kDa
Observed band size: 37 kDa
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands. -
ICC/IF image of ab71970 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71970, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab71970 被引用在 3 文献中.
- Toki S et al. The survival and proliferation of osteosarcoma cells are dependent on the mitochondrial BIG3-PHB2 complex formation. Cancer Sci 112:4208-4219 (2021). PubMed: 34363714
- Sircoulomb F et al. ZNF703 gene amplification at 8p12 specifies luminal B breast cancer. EMBO Mol Med 3:153-66 (2011). ICC/IF ; Human . PubMed: 21328542
- He B et al. A repressive role for prohibitin in estrogen signaling. Mol Endocrinol 22:344-60 (2008). WB, IP ; Mouse . PubMed: 17932104