Anti-RBPMS抗体(ab194213)
Key features and details
- Rabbit polyclonal to RBPMS
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-RBPMS抗体
参阅全部 RBPMS 一抗 -
描述
兔多克隆抗体to RBPMS -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Rat, Human
预测可用于: Pig -
免疫原
Synthetic peptide within Rat RBPMS (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
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阳性对照
- WB: Wild-type HeLa, RT-4 and Rat heart cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.03% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab194213于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Predicted molecular weight: 22 kDa.
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说明 |
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WB
1/1000. Predicted molecular weight: 22 kDa. |
靶标
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功能
Acts as a coactivator of transcriptional activity. Required to increase TGFB1/Smad-mediated transactivation. Acts through SMAD2, SMAD3 and SMAD4 to increase transcriptional activity. Increases phosphorylation of SMAD2 and SMAD3 on their C-terminal SSXS motif, possibly through recruitment of TGFBR1. Promotes the nuclear accumulation of SMAD2, SMAD3 and SMAD4 proteins. Binds to poly(A) RNA. -
组织特异性
Ubiquitously expressed, at various levels depending on the isoform and the tissue. -
序列相似性
Contains 1 RRM (RNA recognition motif) domain. -
结构域
The RRM domain is necessary for interaction with SMAD4. Both the RRM domain and the C-terminus are required for TGFB1/Smad-mediated transactivation activity. -
细胞定位
Nucleus. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 11030 Human
- Entrez Gene: 498642 Rat
- Omim: 601558 Human
- SwissProt: Q93062 Human
- Unigene: 334587 Human
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别名
- FLJ32971 antibody
- Heart and RRM expressed sequence antibody
- Hermes antibody
see all
图片
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All lanes : Anti-RBPMS antibody (ab194213) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : RBPMS Knockout HeLa cell lysate
Lane 3 : RT-4 cell lysate
Lane 4 : U-251 MG cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab194213 observed at 30 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab194213 was shown to react with RBPMS in wild-type HeLa cells in western blot. Here no band was observed in the RBPMS knockout cell line ab264697 (RBPMS knockout cell lysate ab258631), but other antibodies have shown bands in this lysate below 30 kDa that may represent truncated forms and cleaved fragments. This has not been investigated further. HeLa wild-type and RBPMS knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab194213 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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Anti-RBPMS antibody (ab194213) at 1/1000 dilution + Rat heart lysate
Predicted band size: 22 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (25)
ab194213 被引用在 25 文献中.
- Gao J et al. Differential effects of experimental glaucoma on intrinsically photosensitive retinal ganglion cells in mice. J Comp Neurol 530:1494-1506 (2022). PubMed: 34958682
- Beier C et al. Projections of ipRGCs and conventional RGCs to retinorecipient brain nuclei. J Comp Neurol 529:1863-1875 (2021). PubMed: 33104235
- Zhang X et al. Trilogy Development of Proopiomelanocortin Neurons From Embryonic to Adult Stages in the Mice Retina. Front Cell Dev Biol 9:718851 (2021). PubMed: 34676208
- Zeng L et al. RNA-Seq Analysis Reveals an Essential Role of the Tyrosine Metabolic Pathway and Inflammation in Myopia-Induced Retinal Degeneration in Guinea Pigs. Int J Mol Sci 22:N/A (2021). PubMed: 34830490
- Lou X et al. Polydopamine nanoparticles attenuate retina ganglion cell degeneration and restore visual function after optic nerve injury. J Nanobiotechnology 19:436 (2021). PubMed: 34930292