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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation

Anti-RBBP7抗体(ab3535)

  • Datasheet
Reviews (1)Q&A (3)References (13)

Product price, shipping and contact information

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Abpromise

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Western blot - Anti-RBBP7 antibody (ab3535)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBBP7 antibody (ab3535)
  • Immunocytochemistry - Anti-RBBP7 antibody (ab3535)

Key features and details

  • Rabbit polyclonal to RBBP7
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-RBBP7抗体
    参阅全部 RBBP7 一抗
  • 描述

    兔多克隆抗体to RBBP7
  • 宿主

    Rabbit
  • 经测试应用

    适用于: ICC, WB, IHC-Pmore details
  • 种属反应性

    与反应: Human
    预测可用于: Rat, Chicken, Xenopus laevis
  • 免疫原

    Synthetic peptide corresponding to Human RBBP7 aa 1-13.
    Sequence:

    MASKEMFEDTVEEC


    (Peptide available as ab5013)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • Primary antibody说明

    Retinoblastoma protein (Rb) associated protein 46 (RbAp 46) is a nuclear protein that has been shown to interact directly with retinoblastoma protein and is a component of the mSin3 corepressor complex as are the histone deacetylase proteins (HDACs). RbAp 46 shares about 75% sequence identity with the closely related RbAp 48, and appears to be functionally related to the yeast MSI 1 protein in that overexpression of RbAp 46 suppresses heat shock sensitivity in a RAS pathway dependent manner. RbAp 46 has also been demonstrated to be up-regulated by the Wilms’ tumor suppressor gene product, WT1. Additionally, cells transfected with RbAp46 have shown strong inhibition of growth.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • ISWI/NURF/CHRAC
    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Polycomb Silencing
    • PRC2
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HAT
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Class I

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human RBBP7 protein (ab114396)

应用

Our Abpromise guarantee covers the use of ab3535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent concentration.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 47.6 kDa).
IHC-P Use a concentration of 1 µg/ml.

靶标

  • 功能

    Core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the type B histone acetyltransferase (HAT) complex, which is required for chromatin assembly following DNA replication; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; and the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
  • 序列相似性

    Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.
    Contains 7 WD repeats.
  • 细胞定位

    Nucleus.
  • Target information above from: UniProt accession Q16576 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 5931 Human
    • Entrez Gene: 83712 Rat
    • Entrez Gene: 380056 Xenopus laevis
    • Omim: 300825 Human
    • SwissProt: Q16576 Human
    • SwissProt: Q71UF4 Rat
    • SwissProt: Q8AVH1 Xenopus laevis
    • Unigene: 495755 Human
    • Unigene: 3600 Rat
    • Unigene: 55215 Xenopus laevis
    see all
  • 别名

    • G1/S transition control protein binding protein RbAp46 antibody
    • Histone acetyltransferase type B subunit 2 antibody
    • Histone binding protein RBBP7 antibody
    • Histone-binding protein RBBP7 antibody
    • MGC138867 antibody
    • MGC138868 antibody
    • Nucleosome remodeling factor subunit RBAP46 antibody
    • Nucleosome-remodeling factor subunit RBAP46 antibody
    • RBAP46 antibody
    • RBBP 7 antibody
    • RBBP-7 antibody
    • RBBP7 antibody
    • RBBP7_HUMAN antibody
    • Retinoblastoma binding protein 7 antibody
    • Retinoblastoma binding protein p46 antibody
    • Retinoblastoma-binding protein 7 antibody
    • Retinoblastoma-binding protein p46 antibody
    • Retinoblastoma-binding protein RbAp46 antibody
    see all

图片

  • Western blot - Anti-RBBP7 antibody (ab3535)
    Western blot - Anti-RBBP7 antibody (ab3535)
    All lanes : Anti-RBBP7 antibody (ab3535) at 0.5 µg/ml

    Lane 1 : Cell extract prepared from DU-145 2 cells
    Lane 2 : Cell extract prepared from LNCaP cells
    Lane 3 : Cell extract prepared from HepG2 cells

    Predicted band size: 47.6 kDa



    Western blot of RBBP7 on 1) Du-148 2) LNCaP and 3) Hep-G2 cell extracts using ab3535.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBBP7 antibody (ab3535)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBBP7 antibody (ab3535)

    ab3535 staining RBBP7 in Hela cells by Immunocytochemistry/ immunofluorescence. 

  • Immunocytochemistry - Anti-RBBP7 antibody (ab3535)
    Immunocytochemistry - Anti-RBBP7 antibody (ab3535)This image is courtesy of Petra Hajkova, University of Cambridge

    Mouse embryonic fibroblasts (MEFs) were fixed in 4%PFA, permeabilised in PBS 1%BSA 0.1%TX-100 and incubated with 3.3 ug/ml ab3535 (anti-RbAp46 antibody) o/n. The antibody shows nuclear staining.

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
  • 文献 (13)

    发表研究结果有使用 ab3535?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3535 被引用在 13 文献中.

    • Zhang N  et al. High expression of EZH2 as a marker for the differential diagnosis of malignant and benign myogenic tumors. Sci Rep 8:12331 (2018). PubMed: 30120321
    • Moody RR  et al. Probing the interaction between the histone methyltransferase/deacetylase subunit RBBP4/7 and the transcription factor BCL11A in epigenetic complexes. J Biol Chem 293:2125-2136 (2018). PubMed: 29263092
    • Link S  et al. PWWP2A binds distinct chromatin moieties and interacts with an MTA1-specific core NuRD complex. Nat Commun 9:4300 (2018). PubMed: 30327463
    • Hoffmeister H  et al. CHD3 and CHD4 form distinct NuRD complexes with different yet overlapping functionality. Nucleic Acids Res 45:10534-10554 (2017). PubMed: 28977666
    • Balboula AZ  et al. Knockdown of RBBP7 unveils a requirement of histone deacetylation for CPC function in mouse oocytes. Cell Cycle 13:600-11 (2014). PubMed: 24317350
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-4 of 4 Abreviews or Q&A

    Question

    To some one may concern,
    I receive the email from Abcam for 100% Testing Discount of antibody.
    1) Antibody:
    I am interested to testing the antibody:
    a. Anti-RbAp48 antibody [11G10] (ab488)
    b. [and, if possible to get another free, the Anti-RbAp46
    antibody (ab3535) as well]
    2) application
    I am going to test them in WB first. If it works, they will
    be tested in CHIP assay as well.
    3) samples
    Our lab is a neuroscience lab and using broiler chicken as
    animal model. So we will test both of them in broiler chicken brain
    samples.
    Thank you very much.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 03 2012

    Answer

    Thank you very much for your interest in ab488. I am not sure whether you are interested in receiving more information about our testing program or if you are ready to receive the discount code, so I am sending all of the information about the program just in case you are still deciding.
    Since ab488 has not been tested with chicken samples, I can offer a discount off a future purchase if you buy this antibody now, test it in chicken and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.
    If you are interested in this offer, please follow these steps:
    1. Reply to this e-mail to let me know that you would like to proceed and test this antibody. I will then send a discount code.
    2. Purchase ab488 either by phone, fax, or online (www.abcam.com).
    3. Test it in chicken.
    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
    Please remember that submission of the Abreview is sufficient for the discount code to become active.
    We are always pleased to obtain feedback about our products and any information is greatly appreciated!
    Please let me know if you have any questions about this offer and I would be happy to help you further.
    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    回复于 Jul 03 2012

    Western blot abreview for Anti-RbAp46 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (SW480, colorectal cancer)
    Loading amount
    30 µg
    Specification
    SW480, colorectal cancer
    Gel Running Conditions
    Reduced Denaturing (12%/4%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 12 2008

    Question

    I am writing to inquire whether both, or either, of these antibodies (ab86 and ab488, mouse monoclonal antibodies against RbAp48) cross react with RbAp46 protein? I am looking for an antibody that will be specific for RbAp48 only. Also, does the the rabbit polyclonal antibody against RbAp46, ab3535, cross react with RbAp48 protein? Thank You

    Read More

    Abcam community

    Verified customer

    Asked on May 08 2006

    Answer

    Thank you for your patience. The originator of ab3535 has said that the antibody should not cross react with the RbAP48 protein. Please contact us again if you have any additional questions.

    Read More

    Abcam Scientific Support

    回复于 May 15 2006

    Question

    I am writing to inquire whether both, or either, of these antibodies (ab86 and ab488, mouse monoclonal antibodies against RbAp48) cross react with RbAp46 protein? I am looking for an antibody that will be specific for RbAp48 only. Also, does the the rabbit polyclonal antibody against RbAp46, ab3535, cross react with RbAp48 protein? Thank You

    Read More

    Abcam community

    Verified customer

    Asked on May 08 2006

    Answer

    Thank you for your enquiry. For ab86 and ab488, the antibodies have not been tested against RbAp46 so we do not know whether or not the antibodies would react with it. I'm still waiting for information on ab3535 and will let you know when I receive.

    Read More

    Abcam Scientific Support

    回复于 May 10 2006

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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