Anti-RBBP7抗体(ab3535)
Key features and details
- Rabbit polyclonal to RBBP7
- Suitable for: ICC, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RBBP7抗体
参阅全部 RBBP7 一抗 -
描述
兔多克隆抗体to RBBP7 -
宿主
Rabbit -
经测试应用
适用于: ICC, WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Rat, Chicken, Xenopus laevis
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免疫原
Synthetic peptide corresponding to Human RBBP7 aa 1-13.
Sequence:MASKEMFEDTVEEC
(Peptide available asab5013) -
常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
Retinoblastoma protein (Rb) associated protein 46 (RbAp 46) is a nuclear protein that has been shown to interact directly with retinoblastoma protein and is a component of the mSin3 corepressor complex as are the histone deacetylase proteins (HDACs). RbAp 46 shares about 75% sequence identity with the closely related RbAp 48, and appears to be functionally related to the yeast MSI 1 protein in that overexpression of RbAp 46 suppresses heat shock sensitivity in a RAS pathway dependent manner. RbAp 46 has also been demonstrated to be up-regulated by the Wilms’ tumor suppressor gene product, WT1. Additionally, cells transfected with RbAp46 have shown strong inhibition of growth. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab3535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | Use at an assay dependent concentration. | |
| WB | Use a concentration of 0.5 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 47.6 kDa). | |
| IHC-P | Use a concentration of 1 µg/ml. |
靶标
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功能
Core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the type B histone acetyltransferase (HAT) complex, which is required for chromatin assembly following DNA replication; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; and the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex. -
序列相似性
Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.
Contains 7 WD repeats. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5931 Human
- Entrez Gene: 83712 Rat
- Entrez Gene: 380056 Xenopus laevis
- Omim: 300825 Human
- SwissProt: Q16576 Human
- SwissProt: Q71UF4 Rat
- SwissProt: Q8AVH1 Xenopus laevis
- Unigene: 495755 Human
see all -
别名
- G1/S transition control protein binding protein RbAp46 antibody
- Histone acetyltransferase type B subunit 2 antibody
- Histone binding protein RBBP7 antibody
see all
图片
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Western blot - Anti-RBBP7 antibody (ab3535)All lanes : Anti-RBBP7 antibody (ab3535) at 0.5 µg/ml
Lane 1 : Cell extract prepared from DU-145 2 cells
Lane 2 : Cell extract prepared from LNCaP cells
Lane 3 : Cell extract prepared from HepG2 cells
Predicted band size: 47.6 kDaWestern blot of RBBP7 on 1) Du-148 2) LNCaP and 3) Hep-G2 cell extracts using ab3535.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBBP7 antibody (ab3535)ab3535 staining RBBP7 in Hela cells by Immunocytochemistry/ immunofluorescence.
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Immunocytochemistry - Anti-RBBP7 antibody (ab3535)This image is courtesy of Petra Hajkova, University of CambridgeMouse embryonic fibroblasts (MEFs) were fixed in 4%PFA, permeabilised in PBS 1%BSA 0.1%TX-100 and incubated with 3.3 ug/ml ab3535 (anti-RbAp46 antibody) o/n. The antibody shows nuclear staining.
实验方案
数据表及文件
文献 (13)
ab3535 被引用在 13 文献中.
- Zhang N et al. High expression of EZH2 as a marker for the differential diagnosis of malignant and benign myogenic tumors. Sci Rep 8:12331 (2018). PubMed: 30120321
- Moody RR et al. Probing the interaction between the histone methyltransferase/deacetylase subunit RBBP4/7 and the transcription factor BCL11A in epigenetic complexes. J Biol Chem 293:2125-2136 (2018). PubMed: 29263092
- Link S et al. PWWP2A binds distinct chromatin moieties and interacts with an MTA1-specific core NuRD complex. Nat Commun 9:4300 (2018). PubMed: 30327463
- Hoffmeister H et al. CHD3 and CHD4 form distinct NuRD complexes with different yet overlapping functionality. Nucleic Acids Res 45:10534-10554 (2017). PubMed: 28977666
- Balboula AZ et al. Knockdown of RBBP7 unveils a requirement of histone deacetylation for CPC function in mouse oocytes. Cell Cycle 13:600-11 (2014). PubMed: 24317350
