Anti-RBBP7抗体(ab3535)
Key features and details
- Rabbit polyclonal to RBBP7
- Suitable for: ICC, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RBBP7抗体
参阅全部 RBBP7 一抗 -
描述
兔多克隆抗体to RBBP7 -
宿主
Rabbit -
经测试应用
适用于: ICC, WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Rat, Chicken, Xenopus laevis
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免疫原
Synthetic peptide corresponding to Human RBBP7 aa 1-13.
Sequence:MASKEMFEDTVEEC
(Peptide available asab5013)
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
Retinoblastoma protein (Rb) associated protein 46 (RbAp 46) is a nuclear protein that has been shown to interact directly with retinoblastoma protein and is a component of the mSin3 corepressor complex as are the histone deacetylase proteins (HDACs). RbAp 46 shares about 75% sequence identity with the closely related RbAp 48, and appears to be functionally related to the yeast MSI 1 protein in that overexpression of RbAp 46 suppresses heat shock sensitivity in a RAS pathway dependent manner. RbAp 46 has also been demonstrated to be up-regulated by the Wilms’ tumor suppressor gene product, WT1. Additionally, cells transfected with RbAp46 have shown strong inhibition of growth. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab3535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | Use at an assay dependent concentration. | |
| WB | Use a concentration of 0.5 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 47.6 kDa). | |
| IHC-P | Use a concentration of 1 µg/ml. |
靶标
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功能
Core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the type B histone acetyltransferase (HAT) complex, which is required for chromatin assembly following DNA replication; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; and the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex. -
序列相似性
Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.
Contains 7 WD repeats. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5931 Human
- Entrez Gene: 83712 Rat
- Entrez Gene: 380056 Xenopus laevis
- Omim: 300825 Human
- SwissProt: Q16576 Human
- SwissProt: Q71UF4 Rat
- SwissProt: Q8AVH1 Xenopus laevis
- Unigene: 495755 Human
see all -
别名
- G1/S transition control protein binding protein RbAp46 antibody
- Histone acetyltransferase type B subunit 2 antibody
- Histone binding protein RBBP7 antibody
see all
图片
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Western blot - Anti-RBBP7 antibody (ab3535)All lanes : Anti-RBBP7 antibody (ab3535) at 0.5 µg/ml
Lane 1 : Cell extract prepared from DU-145 2 cells
Lane 2 : Cell extract prepared from LNCaP cells
Lane 3 : Cell extract prepared from HepG2 cells
Predicted band size: 47.6 kDaWestern blot of RBBP7 on 1) Du-148 2) LNCaP and 3) Hep-G2 cell extracts using ab3535.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBBP7 antibody (ab3535)ab3535 staining RBBP7 in Hela cells by Immunocytochemistry/ immunofluorescence.
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Immunocytochemistry - Anti-RBBP7 antibody (ab3535)This image is courtesy of Petra Hajkova, University of CambridgeMouse embryonic fibroblasts (MEFs) were fixed in 4%PFA, permeabilised in PBS 1%BSA 0.1%TX-100 and incubated with 3.3 ug/ml ab3535 (anti-RbAp46 antibody) o/n. The antibody shows nuclear staining.
实验方案
数据表及文件
文献 (13)
ab3535 被引用在 13 文献中.
- Zhang N et al. High expression of EZH2 as a marker for the differential diagnosis of malignant and benign myogenic tumors. Sci Rep 8:12331 (2018). PubMed: 30120321
- Moody RR et al. Probing the interaction between the histone methyltransferase/deacetylase subunit RBBP4/7 and the transcription factor BCL11A in epigenetic complexes. J Biol Chem 293:2125-2136 (2018). PubMed: 29263092
- Link S et al. PWWP2A binds distinct chromatin moieties and interacts with an MTA1-specific core NuRD complex. Nat Commun 9:4300 (2018). PubMed: 30327463
- Hoffmeister H et al. CHD3 and CHD4 form distinct NuRD complexes with different yet overlapping functionality. Nucleic Acids Res 45:10534-10554 (2017). PubMed: 28977666
- Balboula AZ et al. Knockdown of RBBP7 unveils a requirement of histone deacetylation for CPC function in mouse oocytes. Cell Cycle 13:600-11 (2014). PubMed: 24317350
