Application
Western blot
Sample
Human Cell lysate - whole cell (LCL)
Specification
LCL
Blocking step
Other as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 10%
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: ECL Anti-rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 55 kDa Non-specific: 40 kDa
Additional data
Additional Notes
The blocking agent we used was Roti-Block from Carl Roth GmbH in Karlsruhe, Germany (A151.1).
As molecular weight marker we used the Full-Range Rainbow Molecular Weight Marker from Amersham Biosciences (RPN800).
As molecular weight marker we used the Full-Range Rainbow Molecular Weight Marker from Amersham Biosciences (RPN800).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Hannes Meyer
Verified customer
提交于 Dec 12 2005