大鼠wild-type PC-12 cell line (ab279978)
概述
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产品名称
大鼠wild-type PC-12 cell line
参阅全部 PC12 细胞裂解液 -
Parental Cell Line
PC12 -
Organism
Rat -
Passage number
<20 -
Biosafety level
1 -
常规说明
Wild-type cell lines are sold with knockout cell lines only - not available for individual purchase.
Cryopreservation cell medium: Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture medium: RPMI + 10% FBS
Initial handling guidelines: Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water for bath approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 5x105-1x106 cells/mL. Seeding density is given as a guide only and should be scaled to align with individual lab schedules.
4. Incubate the culture at 37°C incubator with 5% CO2. Cultures should be monitored daily.Subculture guidelines:
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 5x105-1x106 cells/mL is recommended.
- Subculture when cell density reaches 2-4x106 cells/mL.
We will provide viable cells that proliferate on revival.
性能
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Number of cells
1 x 106 cells/vial, 1 mL -
Tissue
Adrenal -
Cell type
adrenal pheochromocytoma -
Gender
Male -
Mycoplasma free
Yes -
存放说明
Shipped on Dry Ice. Store in liquid nitrogen. -
存储溶液
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether
靶标
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相关性
Generated by Greene and Tischler (1976) from a transplantable rat adrenal pheochromocytoma line, PC12 cells respond reversibly to nerve growth factor (NGF) and therefore have a neuronal phenotype, making the line a useful model system for neuronal differentiation. They produce catecholamines (dopamine, norepinephrine), neurotransmitters, NPP and are therefore used in studies of differentiation, neurobiochemistry and neurobiology.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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