大鼠TIMP1 ELISA试剂盒(ab100782)
Key features and details
- Sensitivity: 4 pg/ml
- Range: 4.1 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
概述
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产品名称
大鼠TIMP1 ELISA试剂盒
参阅全部 TIMP1 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 4 pg/ml -
范围
4.1 pg/ml - 3000 pg/ml -
回收率
93 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 93.26 86% - 104% Serum 92.79 85% - 104% Plasma 94.65 84% - 103% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Rat -
产品概述
Abcam’s TIMP1 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat TIMP1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for rat TIMP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat TIMP- 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-rat TIMP1 2 vials Recombinant rat TIMP1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TIMP1 Microplate (12 x 8 wells) 1 unit TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. -
序列相似性
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
翻译后修饰
The activity of TIMP1 is dependent on the presence of disulfide bonds. -
细胞定位
Secreted. - Information by UniProt
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别名
- Clgi
- Collagenase inhibitor
- Collagenase inhibitor, Human
see all -
数据库链接
- Entrez Gene: 116510 Rat
- SwissProt: P30120 Rat
- Unigene: 25754 Rat
图片
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Typical data B
Representative standard curve using ab100782
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Typical data ARepresentative standard curve using ab100782
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab100782 尚未被引用在任何文献中。