大鼠Myoglobin ELISA试剂盒(ab157739)
Key features and details
- Sensitivity: 3.365 ng/ml
- Range: 6.25 ng/ml - 200 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
概述
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产品名称
大鼠Myoglobin ELISA试剂盒
参阅全部 Myoglobin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 10% -
样品类型
Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
3.365 ng/ml -
范围
6.25 ng/ml - 200 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum > 85 % - % -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Rat -
产品概述
Abcam’s Myoglobin Rat ELISA Kit is a two-site enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Myoglobin levels in rat serum and plasma.
In this assay the Myoglobin present in samples reacts with the anti-Myoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Myoglobin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Myoglobin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Myoglobin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Myoglobin in the test sample. The quantity of Myoglobin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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平台
Microplate
性能
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存放说明
Please refer to protocols. -
组件 1 x 96 tests 100X HRP-conjugated anti-rat Myoglobin antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml Rat Myoglobin Calibrator (lyophilized) 1 vial Rat Myoglobin ELISA Microplate 1 unit Stop Solution 1 x 12ml -
研究领域
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功能
Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. -
序列相似性
Belongs to the globin family. - Information by UniProt
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别名
- MB
- MGC13548
- MYG_HUMAN
- Myoglobin
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数据库链接
- Entrez Gene: 59108 Rat
- SwissProt: Q9QZ76 Rat
- Unigene: 40511 Rat
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab157739 被引用在 1 文献中.
- Zuo Q et al. Response of macrophages in rat skeletal muscle after eccentric exercise. Chin J Traumatol 21:88-95 (2018). PubMed: 29550244