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大鼠MMP8 ELISA试剂盒(ab100779)

Submit a review Q&A (3)References (1)
Typical data B
  • Typical data A

Key features and details

  • Sensitivity: 50 pg/ml
  • Range: 0.05 ng/ml - 40 ng/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Rat

概述

  • 产品名称

    大鼠MMP8 ELISA试剂盒
    参阅全部 MMP8 试剂盒

  • 检测方法

    Colorimetric

  • 样品类型

    Cell culture supernatant, Serum, Plasma

  • 检测类型

    Sandwich (quantitative)

  • 灵敏度

    < 50 pg/ml

  • 范围

    0.05 ng/ml - 40 ng/ml

  • 回收率

    100 %

    特定样本回收率
    样品类型 平均% 范围
    Cell culture supernatant 95.42 83% - 108%
    Serum 109.78 99% - 118%
    Plasma 107.6 98% - 118%

  • 实验步骤

    Multiple steps standard assay

  • 种属反应性

    与反应: Rat

  • 产品概述

    Abcam’s MMP8 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat MMP8 in serum, plasma (using heparin as an anticoagulant, EDTA and Citrate are not recommended), and cell culture supernatants.

    This assay employs an antibody specific for MMP8 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat MMP8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 平台

    Microplate

性能

图片

  • Typical data B
    Typical data B

    Representative standard curve using ab100779

  • Typical data A
    Typical data A

    Representative standard curve using ab100779

文献 (1)

发表研究结果有使用 ab100779？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab100779 被引用在 1 文献中.

  • Monaghan M  et al. A collagen-based scaffold delivering exogenous microrna-29B to modulate extracellular matrix remodeling. Mol Ther 22:786-96 (2014). PubMed: 24402185

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

Question

ab100779, ab100609: can cross-react withmouse?
info on testing discount program

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Answer

Thank you for your patience.

The lab let me know the following:

ab100609 : Cross reactivity with mouse is unknown. If it helps, the capture antibody is monoclonal and the detection antibody is polyclonal.

ab100779 : Untested with mouse, but expected strong cross reactivity based on sequence similarity. Homology of MMP-8 between rat and mouse is ˜91%



If you like to try the rat ELISA kit on mouse samples, you can use our testing discount program I had sent you information about. If you like to participate, please let me know and I'd be happy to send youa discount code.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

ab100779, ab100609: can cross-react withmouse?
info on testing discount program

Read More
Answer

Thank you for contacting us.

I have contacted the lab regarding the cross-reactivity of the 2 kits and will let you know what I find out.

As for the testing discount program, we have the following general information:

For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody/protein/kit at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
This programapplies to ELISA kits and other kits we offer.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Question

Dear,

If your kit below can measure activity MMP-8


MMP8 Rat ELISA Kit - 1 x 96 Well Plate (ab100779)

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Answer

Thank you for your enquiry.

ab100779 does not measure MMP8 activity. It is a sandwich ELISA assay that just quantitates amount of protein present.

I hope this is helpful. Please contact me again if you have any further questions.

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