Key features and details
- Sensitivity: 15 pg/ml
- Range: 24.67 pg/ml - 18000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
参阅全部 MCP1 试剂盒
样品类型Cell culture supernatant, Serum, Plasma
灵敏度< 15 pg/ml
范围24.67 pg/ml - 18000 pg/ml
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 101.4 86% - 105% Serum 95.39 84% - 103% Plasma 92.44 83% - 103%
实验步骤Multiple steps standard assay
Abcam’s MCP1 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat MCP1 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for rat MCP1 coated on a 96-well plate. Standards and samples are pipette into the wells and MCP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat MCP1 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
存放说明Store at -20°C. Please refer to protocols.
组件 1 x 96 tests 100X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Rat MCP1 2 vials MCP1 Microplate (12 x 8 wells) 1 unit Recombinant rat MCP1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
功能Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
序列相似性Belongs to the intercrine beta (chemokine CC) family.
翻译后修饰Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
- Information by UniProt
- C-C motif chemokine 2
ab100777 被引用在 3 文献中.
- Tang W et al. Lipocalin 2 Suppresses Ocular Inflammation by Inhibiting the Activation of NF-?ß Pathway in Endotoxin-Induced Uveitis. Cell Physiol Biochem 46:375-388 (2018). PubMed: 29590655
- Lu YZ et al. Photobiomodulation with 670 nm light ameliorates Müller cell-mediated activation of microglia and macrophages in retinal degeneration. Exp Eye Res 165:78-89 (2017). PubMed: 28888911
- Lu HJ et al. Ruscogenin protects against high-fat diet-induced nonalcoholic steatohepatitis in hamsters. Planta Med 80:870-9 (2014). PubMed: 25116118