大鼠Lumican ELISA试剂盒(ab213917)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
概述
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产品名称
大鼠Lumican ELISA试剂盒
参阅全部 Lumican 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% 1 16 202pg/ml 13.33 = 6.6% 2 16 1179pg/ml 54.23 = 4.6% 3 16 5476pg/ml 339.51 = 6.2% 批次间 样品 n Mean SD CV% 1 24 186pg/ml 16.65 = 8.2% 2 24 1201pg/ml 58.84 = 4.9% 3 24 5528pg/ml 414.6 = 7.5% -
样品类型
Cell culture supernatant, Serum, Hep Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 10 pg/ml -
范围
156 pg/ml - 10000 pg/ml -
检测时间
3h 30m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Rat -
产品概述
The Rat Lumican Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213917) is designed for the quantitative measurement of Rat Lumican in cell culture supernatants, serum and plasma (heparin).
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Lumican has been precoated onto 96-well plates. Standards and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for Lumican is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Lumican amount of sample captured in plate.
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说明
Lumican, also known as LUM, is a protein which in humans is encoded by the LUM gene. This gene is a member of the small interstitial proteoglycan gene (SIPG) family, and it is a keratan sulfate proteoglycan which presents large quantities in the corneal stroma and in interstitial collagenous matrices of the heart, aorta, skeletal muscle, skin, and intervertebral discs. Lumican is mapped to 12q21.33, it interacts with collagen and limits growth of fibrils in diameter. In the cornea, Lumican not only interacts with collagen molecules to limit fibril growth, but also plays a critical role in the regular spacing of fibrils and acquisition of corneal transparency by virtue of its keratan sulfate containing glycosaminoglycan side chains LDC.
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平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 标识符 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-rat Lumican coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti- rat Lumican antibody 1 x 100µl Lyophilized recombinant rat Lumican standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
研究领域
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组织特异性
Cornea and other tissues. -
序列相似性
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
Contains 11 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain. -
发展阶段
Present in the extracellular matrix of human articular cartilage at all ages, although its abundance is far greater in the adult. In the adult cartilage lumican exists predominantly in a glycoprotein form lacking keratan sulfate, whereas the juvenile form of the molecule is a proteoglycan. -
翻译后修饰
Sulfated on tyrosine residue(s). -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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别名
- Keratan sulfate proteoglycan lumican
- KSPG lumican
- LDC
see all -
数据库链接
- Entrez Gene: 81682 Rat
- SwissProt: P51886 Rat
- Unigene: 3087 Rat
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab213917 尚未被引用在任何文献中。