Key features and details
- Sensitivity: 10 pg/ml
- Range: 8.23 pg/ml - 6000 pg/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
参阅全部 IL-10 试剂盒
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12%
样品类型Cell culture extracts, Tissue Extracts
灵敏度< 10 pg/ml
范围8.23 pg/ml - 6000 pg/ml
特定样本回收率 样品类型 平均% 范围 Cell culture extracts 142.1 133% - 149% Tissue Extracts 138.7 127% - 146%
实验步骤Multiple steps standard assay
Abcam’s IL-10 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-10 in cell lysates and tissue lysates.
This assay employs an antibody specific for rat IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
存放说明Store at -20°C. Please refer to protocols.
组件 1 x 96 tests 140X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 2X Cell Lysis Buffer 1 x 5ml 5X Assay Diluent 1 x 15ml 5X Sample Diluent Buffer 1 x 10ml Biotinylated anti-Rat IL-10 2 vials IL-10 Microplate (12 strips x 8 wells) 1 unit Recombinant rat IL-10 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
功能Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
组织特异性Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
序列相似性Belongs to the IL-10 family.
- Information by UniProt
- Cytokine synthesis inhibitory factor
ab100765 被引用在 18 文献中.
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- Tu C et al. Ginsenoside Rg3 alleviates inflammation in a rat model of myocardial infarction via the SIRT1/NF-?B pathway. Exp Ther Med 20:238 (2020). PubMed: 33193843
- Lu HK et al. Pinellia ternata attenuates carotid artery intimal hyperplasia and increases endothelial progenitor cell activity via the PI3K/Akt signalling pathway in wire-injured rats. Pharm Biol 58:1184-1191 (2020). PubMed: 33253601
- Hellenbrand DJ et al. Sustained interleukin-10 delivery reduces inflammation and improves motor function after spinal cord injury. J Neuroinflammation 16:93 (2019). PubMed: 31039819