大鼠IgM,kappa单克隆抗体[RTK2118] -同型对照(ab35768)
Related conjugates and formulations
概述
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产品名称
大鼠IgM,kappa单克隆抗体[RTK2118] -同型对照
参阅全部 Rat 亚类对照 -
经测试应用
适用于: Flow Cyt, WB, IP, ICC/IF, IHC-Fr, IHC-Pmore details -
免疫原
Chemical/ Small Molecule corresponding to Rat. Trinitrophenol + KLH
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常规说明
Isotype Control monoclonal antibodies are used to estimate the non-specific binding of target primary antibodies to cell surface antigens. Non-specific binding is due to Fc receptor binding or other protein-protein interactions. Isotype controls show negligible cross-reactivity with cell surface antigens on tissue sections or in cellular preparations. Isotype Controls should be used at identical concentrations and staining conditions as the target primary antibodies.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Purified IgM -
Isotype control说明
Isotype Control monoclonal antibodies are used to estimate the non-specific binding of target primary antibodies to cell surface antigens. Non-specific binding is due to Fc receptor binding or other protein-protein interactions. Isotype controls show negligible cross-reactivity with cell surface antigens on tissue sections or in cellular preparations. Isotype Controls should be used at identical concentrations and staining conditions as the target primary antibodies. -
克隆
单克隆 -
克隆编号
RTK2118 -
同种型
IgM -
轻链类型
kappa -
研究领域
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别名
- Rat Isotype Control
相关产品
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Alternative Versions
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab35768于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use 2µg for 106 cells.
ab35768 is a negative isotype control |
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WB |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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说明 |
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Flow Cyt
Use 2µg for 106 cells. ab35768 is a negative isotype control |
WB
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
图片
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Overlay histogram showing RAMOS cells stained with ab19795 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19795, 1/10 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat kappa monoclonal [RTK2118] (ab35768, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab35768 被引用在 2 文献中.
- Chandrupatla DMSH et al. Imaging and Methotrexate Response Monitoring of Systemic Inflammation in Arthritic Rats Employing the Macrophage PET Tracer [18F]Fluoro-PEG-Folate. Contrast Media Mol Imaging 2018:8092781 (2018). PubMed: 29681783
- Pereira EM et al. Characterization and phosphoproteomic analysis of a human immortalized podocyte model of Fabry disease generated using CRISPR/Cas9 technology. Am J Physiol Renal Physiol 311:F1015-F1024 (2016). PubMed: 27681560