大鼠IGF-2 ELISA试剂盒(ab213903)
Key features and details
- Sensitivity: 5 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
概述
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产品名称
大鼠IGF-2 ELISA试剂盒
参阅全部 IGF2 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% 1 16 478pg/ml 20.55 4.3% 2 16 1536pg/ml 87.55 5.7% 3 16 2743pg/ml 126.2 4.6% 批次间 样品 n Mean SD CV% 1 24 453pg/ml 30.8 6.8% 2 24 1737pg/ml 140.7 8.1% 3 24 2968pg/ml 222.6 7.5% -
样品类型
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Homogenate -
检测类型
Sandwich (quantitative) -
灵敏度
< 5 pg/ml -
范围
62.5 pg/ml - 4000 pg/ml -
检测时间
3h 30m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Rat
预测可用于: Hamster -
产品概述
The Rat IGF-2 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213903) is designed for the quantitative measurement of Rat IGF-2 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGF-2 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGF-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat IGF-2 amount of sample captured in plate.
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说明
Insulin-like growth factor II (IGF-2) is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is paternally expressed in the fetus and placenta. IGF-2 is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-2 gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues. IGF-2 appears to be induced by placental lactogen during prenatal development. It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.
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平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 标识符 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-rat IGF-2 coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti- Rat IGF-2 antibody 1 x 100µl Lyophilized recombinant Rat IGF-2 standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
研究领域
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功能
The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3. -
疾病相关
Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations. -
序列相似性
Belongs to the insulin family. -
翻译后修饰
O-glycosylated with a core 1 or possibly core 8 glycan. -
细胞定位
Secreted. - Information by UniProt
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别名
- C11orf43
- IGF 2
- IGF II
see all -
数据库链接
- Entrez Gene: 24483 Rat
- SwissProt: P01346 Rat
- Unigene: 118681 Rat
- Unigene: 224460 Rat
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab213903 被引用在 2 文献中.
- Ji F et al. Long non-coding RNA H19 promotes tumorigenesis of ovarian cancer by sponging miR-675. Int J Clin Exp Pathol 12:113-122 (2019). PubMed: 31933725
- Jeon HJ et al. Insulin-like growth factor binding protein-6 released from human mesenchymal stem cells confers neuronal protection through IGF-1R-mediated signaling. Int J Mol Med 40:1860-1868 (2017). PubMed: 29039467