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Anti-RASSF1抗体(ab97749)

Submit a review Q&A (1)References (6)
Western blot - Anti-RASSF1 antibody (ab97749)
  • Immunocytochemistry/ Immunofluorescence - Anti-RASSF1 antibody (ab97749)

Key features and details

  • Rabbit polyclonal to RASSF1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-RASSF1抗体
    参阅全部 RASSF1 一抗

  • 描述

    兔多克隆抗体to RASSF1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, ICC/IFmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Cow

  • 免疫原

    Recombinant fragment corresponding to Human RASSF1 aa 50-350. NP_733832
    Database link: Q9NS23-1

  • 阳性对照

    • A431 and HeLaS3 cell lines. 293T whole cell lysate. HeLa cells

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab97749于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/500 - 1/3000. Predicted molecular weight: 39 kDa.
ICC/IF
1/100 - 1/200.
说明
WB
1/500 - 1/3000. Predicted molecular weight: 39 kDa.
ICC/IF
1/100 - 1/200.

靶标

  • 功能

    Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK4 during Fas-induced apoptosis. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage.

  • 组织特异性

    Isoform A and isoform C are ubiquitously expressed in all tissues tested, however isoform A is absent in many corresponding cancer cell lines. Isoform B is mainly expressed in hematopoietic cells.

  • 序列相似性

    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 Ras-associating domain.
    Contains 1 SARAH domain.

  • 细胞定位

    Nucleus. Predominantly nuclear and Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Nucleus. Localizes to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late telophase in cells. Colocalizes with MDM2 in the nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 123F2 antibody
      • Cardiac specific ras association domain family 1 protein antibody
      • cardiac-specific ras association domain family 1 protein, 123F2 antibody
      • NORE2A antibody
      • OTTHUMP00000195503 antibody
      • OTTHUMP00000195504 antibody
      • OTTHUMP00000195505 antibody
      • OTTHUMP00000195507 antibody
      • Pancreas specific ras association domain family 1 protein antibody
      • Ras association (RalGDS/AF 6) domain family member 1 antibody
      • Ras association domain-containing protein 1 antibody
      • RASF1_HUMAN antibody
      • Rassf1 antibody
      • RASSF1A antibody
      • RDA32 antibody
      • REH3P21 antibody
      • Tumor suppressor protein RDA32 antibody
      • WUGSC:H_LUCA12.5 antibody
      see all

    图片

    • Western blot - Anti-RASSF1 antibody (ab97749)
      Western blot - Anti-RASSF1 antibody (ab97749)
      Anti-RASSF1 antibody (ab97749) at 1/500 dilution + 293T whole cell lysate at 30 µg

      Predicted band size: 39 kDa



      12% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-RASSF1 antibody (ab97749)
      Immunocytochemistry/ Immunofluorescence - Anti-RASSF1 antibody (ab97749)
      Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97749 at 1/100 dilution. Lower image is merged with DNA probe.

    文献 (6)

    发表研究结果有使用 ab97749？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab97749 被引用在 6 文献中.

    • Liang YY  et al. RASSF1A inhibits PDGFB-driven malignant phenotypes of nasopharyngeal carcinoma cells in a YAP1-dependent manner. Cell Death Dis 11:855 (2020). PubMed: 33057010
    • Bao Y  et al. Ras-association domain family 1 (RASSF1A) gene regulates progression, migration and invasion of bladder cancer. Surg Oncol 30:63-71 (2019). PubMed: 31500788
    • Chen T  et al. Circ_0078767 suppresses non-small-cell lung cancer by protecting RASSF1A expression via sponging miR-330-3p. Cell Prolif N/A:e12548 (2018). PubMed: 30507050
    • Li W  et al. Suppressor of hepatocellular carcinoma RASSF1A activates autophagy initiation and maturation. Cell Death Differ N/A:N/A (2018). PubMed: 30315205
    • Hong L  et al. The interaction between miR-148a and DNMT1 suppresses cell migration and invasion by reactivating tumor suppressor genes in pancreatic cancer. Oncol Rep 40:2916-2925 (2018). PubMed: 30226601
    • Bräuer-Hartmann D  et al. PML/RARa-Regulated miR-181a/b Cluster Targets the Tumor Suppressor RASSF1A in Acute Promyelocytic Leukemia. Cancer Res 75:3411-24 (2015). PubMed: 26041820

    客户评价及客户问答

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    Question

    Hi,
    I'm trying doing WB using ab23950, but I could not detect signals around 40KDa in HS68 and BJ5 cells.
    Could you tell me the condition of western blot for this antibody such as which lysis buffer to use, sonication is necessary,
    now much protein per lane, nitrocellulose or PVDF membrane etc.
    Also, which cell type have you tested besides HeLa cells? Do you think it can be observed in fibroblast cells such as HS68,
    BJ cells, NIH3T3 cells?
    Also ab97749 antibody's IF staining in HeLa cells shows nuclear staining where this proteins localization is reported
    as microtubule localization. Why?

    Read More
    Answer

    Thanks for your enquiry.
    We do not have a specific protocol for cell extraction when preparing samples to western blot RASSF1a. We do have a good general WB protocol which you might find useful at https://www.abcam.com/index.html?pageconfig=resource&rid=11375.
    Based on information on the datasheet for ab23950, there is western blot data showing detection of RASSF1a in Hela cells, mouse brain, liver and kidney extract.
    Based on information provided in the SwissProt database, this protein has multiple forms but is expressed in all tissues, but often not in the corresponding tumor cell lines. It may be useful to check the literature on RASSF1a for cell lines that do contain detectable levels.
    Since normal tissues commonly contain detectable levels of RASSF1a, you may be interested in our range of tissue extracts. Simply type in the tissue type into the product search bar at the top of our website, and select 'Lysates' as the product type.
    Also, the SwissProt protein entry for RASSF1a notes that this protein can change cocalization, from cytoplsam to nuclear, depending on the cell cycle. Again, it may be useful to consult the literature for further details.
    I hope this is helpful. Please contact us again if you have any further questions.

    Read More

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