Anti-RASA1抗体[B4F8] (ab2922)
![Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)](https://www.abcam.cn/ps/products/2/ab2922/Images/ab2922-201297-anti-rasa1-antibody-b4f8-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)")
Key features and details
- Mouse monoclonal [B4F8] to RASA1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG2a
概述
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产品名称
Anti-RASA1抗体[B4F8]
参阅全部 RASA1 一抗 -
描述
小鼠单克隆抗体[B4F8] to RASA1 -
宿主
Mouse -
特异性
This antibody is specific for RASA1 (an alternate name of GAP). -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Non human primates
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免疫原
Recombinant full length protein corresponding to Human RASA1.
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表位
Epitope mapping studies suggest that this antibody binds a portion of GAP that contains the src homology regions SH2 and SH3. -
阳性对照
- ICC: C2C12, HeLa, A431; WB: HeLa, A431, mouse brain lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
纯度
Protein A purified -
Primary antibody说明
GTPase Activating Protein (GAP) is capable of stimulating the hydrolysis of GTP by ras p21 proteins, though GAP has little effect on the oncogenic forms of ras. It is also known that several tyrosine kinases such as platelet derived growth factor receptor and epidermal growth factor receptor are involved in the tyrosine phosphorylation of GAP. It has therefore been suggested that GAP may provide a connection or link between growth factor receptors and the ras p21 family. -
克隆
单克隆 -
克隆编号
B4F8 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
Immunofluorescence staining of GAP in mouse fibroblast cells results in diffuse cytoplasmic staining. Following the addition of PDGF, immunofluorescence staining shows that some GAP rapidly translocates to the plasma membrane. |
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| WB |
Use a concentration of 10 µg/ml.
By Western blot, this antibody recognizes a single 120 kDa protein representing ras GAP from RS-2 cell lysate. |
| 说明 |
|---|
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ICC/IF
Use a concentration of 5 µg/ml. Immunofluorescence staining of GAP in mouse fibroblast cells results in diffuse cytoplasmic staining. Following the addition of PDGF, immunofluorescence staining shows that some GAP rapidly translocates to the plasma membrane. |
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WB
Use a concentration of 10 µg/ml. By Western blot, this antibody recognizes a single 120 kDa protein representing ras GAP from RS-2 cell lysate. |
靶标
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功能
Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21. -
组织特异性
In placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast). Not detected in stromal, endothelial or Hofbauer cells (at protein level). -
疾病相关
Note=Mutations in the SH2 domain of RASA seem to be oncogenic and cause basal cell carcinomas.
Defects in RASA1 are the cause of capillary malformation-arteriovenous malformation (CMAVM) [MIM:608354]. CMAVM is a disorder characterized by atypical capillary malformations that are multiple, small, round to oval in shape and pinkish red in color. These capillary malformations are associated with either arteriovenous malformation, arteriovenous fistula, or Parkes Weber syndrome.
Defects in RASA1 are a cause of Parkes Weber syndrome (PKWS) [MIM:608355]. PKWS is a disorder characterized by a cutaneous flush with underlying multiple micro-arteriovenous fistulas, in association with soft tissue and skeletal hypertrophy of the affected limb. -
序列相似性
Contains 1 C2 domain.
Contains 1 PH domain.
Contains 1 Ras-GAP domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
翻译后修饰
The N-terminus is blocked. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 5921 Human
- Entrez Gene: 218397 Mouse
- Omim: 139150 Human
- SwissProt: P20936 Human
- Unigene: 664080 Human
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别名
- CM AVM antibody
- CMAVM antibody
- DKFZp434N071 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)
ab2922 labelling GAP (green) in the cytoplasm of A431 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.
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![Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)](https://www.abcam.cn/ps/products/2/ab2922/Images/ab2922-201296-anti-rasa1-antibody-b4f8-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)ab2922 labelling GAP (green) in the cytoplasm of C2C12 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.
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![Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)](https://www.abcam.cn/ps/products/2/ab2922/Images/ab2922-201295-anti-rasa1-antibody-b4f8-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)ab2922 labelling GAP (green) in the cytoplasm of Hela cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.
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![Western blot - Anti-RASA1 antibody [B4F8] (ab2922)](https://www.abcam.cn/ps/products/2/ab2922/Images/ab2922-201293-anti-rasa1-antibody-b4f8-western-blot.jpg)
Western blot - Anti-RASA1 antibody [B4F8] (ab2922)All lanes : Anti-RASA1 antibody [B4F8] (ab2922) at 1/200 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Mouse brain cell lysate
Lysates/proteins at 25 µg per lane.
Observed band size: 110 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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Datasheet download
文献 (6)
ab2922 被引用在 6 文献中.
- Zhu X et al. miR-188-5p promotes oxaliplatin resistance by targeting RASA1 in colon cancer cells. Oncol Lett 21:481 (2021). PubMed: 33968197
- Chen CC et al. KRAS K104 modification affects the KRASG12D-GEF interaction and mediates cell growth and motility. Sci Rep 10:17447 (2020). PubMed: 33060649
- Bonanomi D et al. p190RhoGAP Filters Competing Signals to Resolve Axon Guidance Conflicts. Neuron 102:602-620.e9 (2019). PubMed: 30902550
- Lei Z et al. MicroRNA-132/212 family enhances arteriogenesis after hindlimb ischaemia through modulation of the Ras-MAPK pathway. J Cell Mol Med 19:1994-2005 (2015). WB, IF . PubMed: 25945589
- Fiedler J et al. MicroRNA-24 regulates vascularity after myocardial infarction. Circulation 124:720-30 (2011). WB, IHC-Fr ; Mouse . PubMed: 21788589
- Settleman J et al. Molecular cloning of cDNAs encoding the GAP-associated protein p190: implications for a signaling pathway from ras to the nucleus. Cell 69:539-49 (1992). PubMed: 1581965
