Anti-RASA1抗体[B4F8] (ab2922)

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Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)
  • Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)
  • Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)
  • Western blot - Anti-RASA1 antibody [B4F8] (ab2922)

Key features and details

  • Mouse monoclonal [B4F8] to RASA1
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG2a

概述

  • 产品名称

    Anti-RASA1抗体[B4F8]
    参阅全部 RASA1 一抗

  • 描述

    小鼠单克隆抗体[B4F8] to RASA1

  • 宿主

    Mouse

  • 特异性

    This antibody is specific for RASA1 (an alternate name of GAP).

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    Human
    WB
    Human
    See all applications and species data

  • 免疫原

    Recombinant full length protein corresponding to Human RASA1.

  • 表位

    Epitope mapping studies suggest that this antibody binds a portion of GAP that contains the src homology regions SH2 and SH3.

  • 阳性对照

    • RS-2 cell lysate. Mouse fibroblast cells.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...

  • 纯度

    Protein A purified

  • Primary antibody说明

    GTPase Activating Protein (GAP) is capable of stimulating the hydrolysis of GTP by ras p21 proteins, though GAP has little effect on the oncogenic forms of ras. It is also known that several tyrosine kinases such as platelet derived growth factor receptor and epidermal growth factor receptor are involved in the tyrosine phosphorylation of GAP. It has therefore been suggested that GAP may provide a connection or link between growth factor receptors and the ras p21 family.

  • 克隆

    单克隆

  • 克隆编号

    B4F8

  • 同种型

    IgG2a

  • 研究领域

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
ICC/IF
Mouse
Human
WB
Human
All applications
Non human primates
应用 Ab评论 说明
ICC/IF
Use a concentration of 5 µg/ml.

Immunofluorescence staining of GAP in mouse fibroblast cells results in diffuse cytoplasmic staining. Following the addition of PDGF, immunofluorescence staining shows that some GAP rapidly translocates to the plasma membrane.
WB
Use a concentration of 10 µg/ml.

By Western blot, this antibody recognizes a single 120 kDa protein representing ras GAP from RS-2 cell lysate.
说明
ICC/IF
Use a concentration of 5 µg/ml.

Immunofluorescence staining of GAP in mouse fibroblast cells results in diffuse cytoplasmic staining. Following the addition of PDGF, immunofluorescence staining shows that some GAP rapidly translocates to the plasma membrane.
WB
Use a concentration of 10 µg/ml.

By Western blot, this antibody recognizes a single 120 kDa protein representing ras GAP from RS-2 cell lysate.

靶标

  • 功能

    Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21.

  • 组织特异性

    In placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast). Not detected in stromal, endothelial or Hofbauer cells (at protein level).

  • 疾病相关

    Note=Mutations in the SH2 domain of RASA seem to be oncogenic and cause basal cell carcinomas.
    Defects in RASA1 are the cause of capillary malformation-arteriovenous malformation (CMAVM) [MIM:608354]. CMAVM is a disorder characterized by atypical capillary malformations that are multiple, small, round to oval in shape and pinkish red in color. These capillary malformations are associated with either arteriovenous malformation, arteriovenous fistula, or Parkes Weber syndrome.
    Defects in RASA1 are a cause of Parkes Weber syndrome (PKWS) [MIM:608355]. PKWS is a disorder characterized by a cutaneous flush with underlying multiple micro-arteriovenous fistulas, in association with soft tissue and skeletal hypertrophy of the affected limb.

  • 序列相似性

    Contains 1 C2 domain.
    Contains 1 PH domain.
    Contains 1 Ras-GAP domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.

  • 翻译后修饰

    The N-terminus is blocked.

  • 细胞定位

    Cytoplasm.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CM AVM antibody
      • CMAVM antibody
      • DKFZp434N071 antibody
      • GAP antibody
      • GTPase activating protein antibody
      • GTPase-activating protein antibody
      • OTTHUMP00000222390 antibody
      • OTTHUMP00000222391 antibody
      • OTTHUMP00000222392 antibody
      • OTTHUMP00000222393 antibody
      • p120GAP antibody
      • p120RASGAP antibody
      • PKWS antibody
      • Ras GTPase-activating protein 1 antibody
      • RAS p21 protein activator (GTPase activating protein) 1 antibody
      • Ras p21 protein activator antibody
      • RASA antibody
      • RASA1 antibody
      • RASA1_HUMAN antibody
      • RasGAP antibody
      • Triphosphatase activating protein antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)
      Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)

      ab2922 labelling GAP (green) in the cytoplasm of A431 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.

    • Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)
      Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)

      ab2922 labelling GAP (green) in the cytoplasm of C2C12 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.

    • Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)
      Immunocytochemistry/ Immunofluorescence - Anti-RASA1 antibody [B4F8] (ab2922)

      ab2922 labelling GAP (green) in the cytoplasm of Hela cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Bue (DAPI) - nuclei. Images were taken at a magnification of 60x.

    • Western blot - Anti-RASA1 antibody [B4F8] (ab2922)
      Western blot - Anti-RASA1 antibody [B4F8] (ab2922)
      All lanes : Anti-RASA1 antibody [B4F8] (ab2922) at 1/200 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : Mouse brain cell lysate

      Lysates/proteins at 25 µg per lane.

      Observed band size: 110 kDa why is the actual band size different from the predicted?

    文献 (4)

    发表研究结果有使用 ab2922？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab2922 被引用在 4 文献中.

    • Bonanomi D  et al. p190RhoGAP Filters Competing Signals to Resolve Axon Guidance Conflicts. Neuron 102:602-620.e9 (2019). PubMed: 30902550
    • Lei Z  et al. MicroRNA-132/212 family enhances arteriogenesis after hindlimb ischaemia through modulation of the Ras-MAPK pathway. J Cell Mol Med 19:1994-2005 (2015). WB, IF . PubMed: 25945589
    • Fiedler J  et al. MicroRNA-24 regulates vascularity after myocardial infarction. Circulation 124:720-30 (2011). WB, IHC-Fr ; Mouse . PubMed: 21788589
    • Settleman J  et al. Molecular cloning of cDNAs encoding the GAP-associated protein p190: implications for a signaling pathway from ras to the nucleus. Cell 69:539-49 (1992). PubMed: 1581965

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