重组Anti-RAP1A + RAP1B抗体[EPR14815(B)] (ab181858)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14815(B)] to RAP1A + RAP1B
- Suitable for: Flow Cyt (Intra), IP, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-RAP1A + RAP1B抗体[EPR14815(B)]
参阅全部 RAP1A + RAP1B 一抗 -
描述
兔单克隆抗体[EPR14815(B)] to RAP1A + RAP1B -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), IP, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa, A431, 293 and MOLT4 cell lysates; HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR14815(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181858于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/30.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IP |
1/50.
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WB |
1/10000 - 1/50000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
|
说明 |
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Flow Cyt (Intra)
1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IP
1/50. |
WB
1/10000 - 1/50000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
靶标
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相关性
RAP1A and RAP1B belong to a family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between the RAP and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. Human RAP1B is 95% identical to RAP1A. RAP1A and B are proposed to regulate Ras-mediated signalling and may also be involved in the regulation of integrin-mediated cell adhesion, although the mechanism of regulation is not known. -
细胞定位
Cell Membrane; Attached to the membrane by a lipid anchor -
数据库链接
- Entrez Gene: 5906 Human
- Entrez Gene: 5908 Human
- Entrez Gene: 109905 Mouse
- Entrez Gene: 215449 Mouse
- Entrez Gene: 171337 Rat
- Entrez Gene: 295347 Rat
- Omim: 179520 Human
- Omim: RAP1B Human
see all -
别名
- C21KG antibody
- G 22K antibody
- GTP binding protein smg p21A antibody
see all
图片
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All lanes : Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858) at 1/50000 dilution
Lane 1 : Hela cell lysate
Lane 2 : A431 cell lysate
Lane 3 : 293 cell lysate
Lane 4 : MOLT4 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 21 kDa
Observed band size: 21 kDa -
Intracellular Flow Cytometry analysis of paraformaldehyde-fixed HeLa cells labeling RAP1B with ab181858 at a 1/30 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).
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Western blot analysis on immunoprecipitation from 1) 293 cell lysate or 2) negative control, labeling RAP1B using ab181858 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab181858 被引用在 1 文献中.
- Shen Y & Ding W Therapeutic Hypothermia Mitigates the Sepsis-Increased Permeability in EA. hy926 Cells by Preserving Rap1 Expression. Ther Hypothermia Temp Manag 11:201-207 (2021). PubMed: 33709787