Anti-RanBP1抗体(ab2937)
Key features and details
- Rabbit polyclonal to RanBP1
- Suitable for: WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-RanBP1抗体
参阅全部 RanBP1 一抗 -
描述
兔多克隆抗体to RanBP1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICCmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Cow
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免疫原
Synthetic peptide corresponding to Human RanBP1 aa 1-18.
Sequence:MAAAKDTHEDHDTSTENT
(Peptide available asab4938) -
阳性对照
- WB: HeLa, Human brain tissue and NIH-3T3 cell lysate. ICC: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 24 kDa).
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| ICC |
Use a concentration of 2 µg/ml.
|
| 说明 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 24 kDa). |
|
ICC
Use a concentration of 2 µg/ml. |
靶标
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功能
Inhibits GTP exchange on Ran. Forms a Ran-GTP-RANBP1 trimeric complex. Increase GTP hydrolysis induced by the Ran GTPase activating protein RANGAP1. May act in an intracellular signaling pathway which may control the progression through the cell cycle by regulating the transport of protein and nucleic acids across the nuclear membrane. -
序列相似性
Belongs to the RANBP1 family.
Contains 1 RanBD1 domain. - Information by UniProt
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数据库链接
- Entrez Gene: 533251 Cow
- Entrez Gene: 5902 Human
- Entrez Gene: 19385 Mouse
- Omim: 601180 Human
- SwissProt: Q3T0M7 Cow
- SwissProt: P43487 Human
- SwissProt: P34022 Mouse
- Unigene: 24763 Human
see all -
别名
- HTF9A antibody
- RAN binding protein 1 antibody
- Ran specific GTPase activating protein antibody
see all
图片
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Western blot - Anti-RanBP1 antibody (ab2937)All lanes : Anti-RanBP1 antibody (ab2937) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Human brain tissue lysate
Lane 3 : NIH-3T3 cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP conjugated anti-rabbit
Developed using the ECL technique.
Predicted band size: 24 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute -
Immunocytochemistry - Anti-RanBP1 antibody (ab2937)Immunocytochemistry analysis of HeLa cells labeling RanB1 with ab2937 at 2 µg/mL. Cells were fixed in 4% Paraformaldehyde, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 1% BSA for 1 hour at RT. Cells were incubated for 3 hours at RT and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1:2000 dilution for 45 minutes at RT (Panel A: green). Nuclei (Panel B: blue) were stained with DAPI. F-actin (Panel C: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1:300 dilution. Panel D is a merged image showing cytoplasmic localization. Panel E shows no primary antibody control. The images were captured at 60X magnification.
数据表及文件
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Datasheet download
文献 (3)
ab2937 被引用在 3 文献中.
- Prpar Mihevc S et al. TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins. Sci Rep 6:33996 (2016). WB, ICC/IF . PubMed: 27665936
- Štalekar M et al. Proteomic analyses reveal that loss of TDP-43 affects RNA processing and intracellular transport. Neuroscience 293:157-70 (2015). WB, ICC/IF . PubMed: 25743254
- Schulz M et al. Proteomic analysis of peripheral blood mononuclear cells: selective protein processing observed in patients with rheumatoid arthritis. J Proteome Res 6:3752-9 (2007). WB ; Human . PubMed: 17705417
