Key features and details
- Rabbit polyclonal to RAIN
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
参阅全部 RAIN 一抗
经测试应用适用于: WBmore details
预测可用于: Rat, Rabbit, Horse, Cow, Dog, Human, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
corresponding to RAIN.
(Peptide available as
- This antibody gave a positive signal in Mouse Lung tissue lysate.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab129820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250. Detects a band of approximately 115 kDa (predicted molecular weight: 103 kDa).|
功能May act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS-mediated transformation.
组织特异性Highly expressed in heart. Detected at lower levels in placenta and pancreas.
序列相似性Contains 1 dilute domain.
Contains 1 Ras-associating domain.
细胞定位Cytoplasm > perinuclear region. Golgi apparatus > Golgi stack. Associated with perinuclear vesicles. Is recruited to Golgi stacks by activated HRAS.
- Information by UniProt
- FLJ20401 antibody
- Rain antibody
- RAIN protein fragment antibody
Anti-RAIN antibody (ab129820) at 1/250 dilution +
Mouse lung normal tissue lysate - total protein (ab29297) at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 103 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
Additional bands at: 23 kDa, 39 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
The expression profile observed is consistent with what has been described in the literature PMID:15031288. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab129820 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.