Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa cells)
Total protein in input
200 µg
Specification
HeLa cells
Immuno-precipitation step
Protein A/G
Other product details
Concentration
5 µg/mg lysate
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 50mMTris,pH8.0,50mMKCl,1mMEDTA,5%glycero
Additional data
Additional Notes
Mock IP was performed using 5µg/mg lysate DNA Polymerase delta prebleed serum. IP step was done with ProtA/G for 6h at 4C with shaking using 200 µg of HeLa whole cell extract. The relative amounts of protein loaded on the gel were as follows: Input - 20%, FT - 20%, IP - 100%.
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提交于 Apr 08 2011