Key features and details
- Goat polyclonal to Rad51C
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
参阅全部 Rad51C 一抗
特异性This antibody is expected to recognise all three human isoforms.
经测试应用适用于: WBmore details
- HeLa lysate.
常规说明Principal Names – RAD51C; RAD51L2; RAD51 homolog C (S. cerevisiae); yeast RAD51 homolog 3; DNA repair protein RAD51 homolog 3 Official Gene Symbol - RAD51C GenBank Accession Number – NP_002867; NP_478123; NP_478124 LocusLink ID - 5889 (human) Gene Ontology terms - adenosinetriphosphatase activity; DNA binding activity; DNA repair; DNA recombination; nucleus.
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA
Concentration information loading...
纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab3669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).
1 hour primary incubation is recommended for this product.
功能Essential for the homologous recombination (HR) pathway of DNA repair. Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. The RAD51B-RAD51C dimer exhibits single-stranded DNA-dependent ATPase activity. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Participates in branch migration and Holliday junction resolution and thus is important for processing HR intermediates late in the DNA repair process. Also has an early function in DNA repair in facilitating phosphorylation of the checkpoint kinase CHK2 and thereby transduction of the damage signal, leading to cell cycle arrest and HR activation. Protects RAD51 from ubiquitin-mediated degradation that is enhanced following DNA damage. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and XRCC3. Contributes to DNA cross-link resistance, sister chromatid cohesion and genomic stability. Involved in maintaining centrosome number in mitosis.
组织特异性Expressed in a variety of tissues, with highest expression in testis, heart muscle, spleen and prostate.
疾病相关Defects in RAD51C are the cause of Fanconi anemia complementation group O (FANCO) [MIM:613390]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
Defects in RAD51C are the cause of breast-ovarian cancer familial type 3 (BROVCA3) [MIM:613399]. It is a condition associated with familial predisposition to cancer of the breast and ovaries. Characteristic features in affected families are an early age of onset of breast cancer (often before age 50), increased chance of bilateral cancers (cancer that develop in both breasts, or both ovaries, independently), frequent occurrence of breast cancer among men, increased incidence of tumors of other specific organs, such as the prostate.
序列相似性Belongs to the RecA family. RAD51 subfamily.
细胞定位Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Mitochondrion. DNA damage induces an increase in nuclear levels. Accumulates in DNA damage induced nuclear foci or RAD51C foci which is formed during the S or G2 phase of cell cycle. Accumulation at DNA lesions requires the presence of NBN/NBS1, ATM and RPA.
- Information by UniProt
- BROVCA3 antibody
- DNA repair protein RAD51 homolog 3 antibody
- FANCO antibody
ab3669 staining (2
µg/ml) of HeLa lysate (RIPA buffer, 30 µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence. ab3669 staining (2µg/ml) of HeLa lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.
ab3669 被引用在 2 文献中.
- Makkonen H et al. Long-range activation of FKBP51 transcription by the androgen receptor via distal intronic enhancers. Nucleic Acids Res 37:4135-48 (2009). PubMed: 19433513
- Katsura M et al. The ATR-Chk1 pathway plays a role in the generation of centrosome aberrations induced by Rad51C dysfunction. Nucleic Acids Res 37:3959-68 (2009). PubMed: 19403737