Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Glioblastoma cells U87)
Permeabilization
Yes - PBST 0.3%
Specification
Glioblastoma cells U87
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Alexa 488
Host species: Cat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Cat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Small permeabilisation before the blocking of 1min with PBST
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Matthias Dedobbeleer
Verified customer
提交于 Jun 01 2018