Anti-Rad51抗体[51RAD01] (ab1837)

Reviews (9)Q&A (19)References (16)

Key features and details

  • Mouse monoclonal [51RAD01] to Rad51
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Rad51抗体[51RAD01]
    参阅全部 Rad51 一抗

  • 描述

    小鼠单克隆抗体[51RAD01] to Rad51

  • 宿主

    Mouse

  • 经测试应用

    适用于: Flow Cyt, ICC/IFmore details
    不适用于: IHC-P

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    BALB/C mice were injected with recombinant Rad51 protein.

  • 阳性对照

    • Testis

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...

  • 纯度

    IgG fraction

  • 克隆

    单克隆

  • 克隆编号

    51RAD01

  • 同种型

    IgG1

  • 轻链类型

    kappa

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab1837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
ICC/IF 1/10. PMID 18663141
  • 应用说明
    Is unsuitable for IHC-P.

    • 靶标

    • 功能

      Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.

    • 组织特异性

      Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.

    • 疾病相关

      Breast cancer
      Mirror movements 2
      Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers.

    • 序列相似性

      Belongs to the RecA family. RAD51 subfamily.
      Contains 1 HhH domain.

    • 结构域

      The nuclear localization may reside in the C-terminus (between 259 and 339 AA).

    • 翻译后修饰

      Ubiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
      Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.

    • 细胞定位

      Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
    • Information by UniProt

    • 数据库链接

      see all

    • 别名

      • BRCA1/BRCA2 containing complex, subunit 5 antibody
      • BRCC 5 antibody
      • BRCC5 antibody
      • DNA repair protein RAD51 homolog 1 antibody
      • DNA repair protein rhp51 antibody
      • FANCR antibody
      • hRAD51 antibody
      • HsRAD51 antibody
      • HsT16930 antibody
      • MRMV2 antibody
      • Rad 51 antibody
      • RAD51 antibody
      • RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) antibody
      • RAD51 homolog A antibody
      • RAD51 homolog antibody
      • RAD51 recombinase antibody
      • RAD51, S. cerevisiae, homolog of antibody
      • RAD51_HUMAN antibody
      • RAD51A antibody
      • RECA antibody
      • RecA like protein antibody
      • RecA, E. coli, homolog of antibody
      • Recombination protein A antibody
      see all

    图片

    • Flow Cytometry - Anti-Rad51 antibody [51RAD01] (ab1837)
      Flow Cytometry - Anti-Rad51 antibody [51RAD01] (ab1837)

      Overlay histogram showing HeLa cells stained with ab1837 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1837, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was goat anti-mouse DyLight® 488 goat (IgG H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody [51RAD01] (ab1837)
      Immunocytochemistry/ Immunofluorescence - Anti-Rad51 antibody [51RAD01] (ab1837)Image from Shin YH et al., PLoS Genet. 2010 Nov 4;6(11):e1001190.Fig 5.; doi:10.1371/journal.pgen.1001190November 4, 2010, PLoS Genet 6(11): e1001190.

      Immunofluorescence analysis of wild-type (+/+) and Hormad1-/- (-/-) murine (mouse) zygotene spermatocytes, staining Rad51 with ab1837.

    文献 (16)

    发表研究结果有使用 ab1837？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab1837 被引用在 16 文献中.

    • Bodo S  et al. Single-dose radiotherapy disables tumor cell homologous recombination via ischemia/reperfusion injury. J Clin Invest 129:786-801 (2019). PubMed: 30480549
    • Ho TT  et al. Autophagy maintains the metabolism and function of young and old stem cells. Nature 543:205-210 (2017). PubMed: 28241143
    • Ganesan S  et al. Obesity alters phosphoramide mustard-induced ovarian DNA repair in mice. Biol Reprod 96:491-501 (2017). PubMed: 28203708
    • Venkatachalam G  et al. Replication stress-induced endogenous DNA damage drives cellular senescence induced by a sub-lethal oxidative stress. Nucleic Acids Res 45:10564-10582 (2017). PubMed: 28985345
    • Chu WK  et al. FBH1 influences DNA replication fork stability and homologous recombination through ubiquitylation of RAD51. Nat Commun 6:5931 (2015). PubMed: 25585578
    View all Publications for this product

    客户评价及客户问答

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    1-10 of 28 Abreviews or Q&A

    Western blot abreview for Anti-Rad51 antibody [51RAD01]

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Melanoma)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    30 µg
    Specification
    Melanoma
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 100°C
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 19 2019

    ChIP abreview for Anti-Rad51 antibody [51RAD01]

     Good
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - nuclear (293T)
    Negative control
    IgG control
    Specification
    293T
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 15 minute(s) and 0 second(s)
    Specification of the cross-linking agent: PFA
    Positive control
    GAPDH gene
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 02 2019

    Immunoprecipitation abreview for Anti-Rad51 antibody [51RAD01]

     Good
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (293T)
    Total protein in input
    100000 cells
    Immuno-precipitation step
    Protein A/G
    Specification
    293T
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 25 2019

    Western blot abreview for Anti-Rad51 antibody [51RAD01]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hela)
    Gel Running Conditions
    Non-reduced Denaturing (12%)
    Loading amount
    10 µg
    Specification
    Hela
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 21 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Rad51 antibody [51RAD01]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Hela)
    Permeabilization
    Yes - Triton X100
    Specification
    Hela
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 20 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Rad51 antibody [51RAD01]

     Poor
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Planarian Cell (whole animal IF)
    Permeabilization
    Yes - tritonX-100
    Specification
    whole animal IF
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 20 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

     Average
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 0.01M citric acid/citrate sodium
    Sample
    Mouse Tissue sections (mouse testes)
    Specification
    mouse testes
    Permeabilization
    No
    Fixative
    4% PFA
    Read More

    Abcam user community

    Verified customer

    提交于 May 29 2013

    Question

    Dear xxxx,

    Thank you for your email. I hope the experiment will work better with this new vial.

    I will let you know about the outcome in the coming weeks.

    Thank you very much for your prompt replies and for your helpfulness.

    Best regards,

    Read More
    Answer

    That is no problem at all. I am glad to have been of some help. Please do let me know how you get on.

    Read More

    Question

    Dear xxxx,

    I appreciate your helpfulness in trying to solve this problem. I was wondering whether some other customer gave you negative feedback as regards the ab1837 antibody from this batch. If this is not the case, it is more likely that something was wrong only for the aliquote we received. We would like to get a new vial of the same ab1837 antibody and give it one more try. In case we run into the same problem, then we would consider the option of a refund or of buying a different antibody.

    You asked some details to track our order: I have the "purchase number" xxxxx

    In case you need further details about the ordering procedure, please do not hesitate to contact me again.

    Best regards,

    Read More
    Answer

    Thank you for your email. I am sorry i was not able to get back to you yesterday.

    I can confirm we have had no other reports of concerns with the lot of the antibody you have been using. I have therefore arranged for a new vial of this lot to be sent to you. If you have similar problems again, please do let me know. You would of course be covered by the Abpromise and would be entitled to a replacement antibody or refund if the problems persist.

    The order number is xxxxx (Purchase order number xxxxx). If you have any problems receiving this please do let me know.

    I hope this replacement vial performs better for you. I look forward to hearing how you get on.

    Until then, I wish you all the best with your research.

    Read More

    Question

    Dear xxxx,

    As I told you in my previous mail, I repeated the immunostaining following your suggestion of testing different blocking conditions and changing the antibody diluent to a less concentrated BSA solution.

    Unfortunately this only increased my background but the quality of the staining did not increase. It is still not possible to detect RAD51 foci with the ab1837 that we bought. Our secondary antibody is not contributing either to the background staining.

    I think it is of interest for you to know that we tested also ab133534 (rabbit monoclonal) on our preparations in the usual conditions (the ones I described in my first enquiry): this antibody works quite well (1:250) although it gives a little bit too much background compared to our homemade rabbit polyclonal.

    Taking all this together, I would guess that there is really something wrong with the ab1837 that we received.

    You mentioned in your previous reply that you could provide a replacement in case the antibody would still not work. If that is still an option, we would like to proceed that way, also because there are published data showing a quite good staining of RAD51 with ab1837 in testis spread preparations.

    I am looking forward to hearing from you soon

    Kind regards,

    Read More
    Answer

    Thank you for your reply and for letting me know how you have been getting on.

    I am sorry that my suggestions have not improved the results observed. As you say, especially with the positive results with the rabbit monoclonal ab133534, it may be that the vial you received may have lost its activity. I am sorry for the inconvenience this has caused you.

    If you would like I can provide you with a free vial of ab1837 however, we only have the same lot you have been trying in stock. Alternatively I can provide you with a different antibody altogether such as the chicken polyclonal ab63802 or the rabbit polyclonal ab137323. Or if you would prefer, I could provide you with an alternative antibody against any target of your choice (as you already have ab133534 which seems to be working for you) or a credit note or refund.

    In order to arrange for any of these I would need confirmation of the order number on which ab1837 was received. Could you provide this, or if you do not have this information, the approximate delivery date and the delivery address used?

    Many thanks for your cooperation. I look forward to hearing how you would like to proceed.

    Read More

    1-10 of 28 Abreviews or Q&A

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