Name: Anti-Rad51 antibody [14B4]
SKU: ab213
Rating: 4 (9 reviews)

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Western blot - Anti-Rad51 antibody [14B4] (ab213)

Key features and details

Mouse monoclonal [14B4] to Rad51
Suitable for: IHC-P, WB
Reacts with: Human
Isotype: IgG2b

产品名称

Anti-Rad51抗体[14B4]
参阅全部 Rad51 一抗
描述

小鼠单克隆抗体[14B4] to Rad51
宿主

Mouse
特异性

Specific for human Rad51.

Tested Applications & Species

Application	Species
IHC-P	Human
WB	Human

See all applications and species data

免疫原

Recombinant full length protein corresponding to Human Rad51 aa 1-338.
Database link: Q06609
阳性对照
- WB: RAD51 shRNA transfected (+) 293T whole cell extracts, Jurkat, Raji and NCI-H929 whole cell lysate. IHC-P: BT483 xenoograft tissue, human colon esophagus tissue.
常规说明

This product was changed from ascites to tissue culture supernatant on 17^th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

形式

Liquid
存放说明

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
存储溶液

pH: 7.40
Constituent: 100% PBS
Concentration information loading...
纯度

Immunogen affinity purified
克隆

单克隆
克隆编号

14B4
骨髓瘤

NS1
同种型

IgG2b
轻链类型

kappa
研究领域

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Isotype control
- Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
Recombinant Protein
- Recombinant human Rad51 protein (Active) (ab63808)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用	Species
IHC-P	Human
WB	Human

应用	Ab评论	说明
IHC-P	(1)	1/100 - 1/1000.
WB	(5)	1/500 - 1/3000. Predicted molecular weight: 37 kDa. Please see WB protocol in image legend.

说明
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 37 kDa. Please see WB protocol in image legend.

应用说明

Is unsuitable for ICC/IF.

功能

Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
组织特异性

Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
疾病相关

Breast cancer
Mirror movements 2
Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers.
序列相似性

Belongs to the RecA family. RAD51 subfamily.
Contains 1 HhH domain.
结构域

The nuclear localization may reside in the C-terminus (between 259 and 339 AA).
翻译后修饰

Ubiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.
细胞定位

Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
Target information above from: UniProt accession Q06609 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 5888 Human
- Omim: 179617 Human
- SwissProt: Q06609 Human
- Unigene: 631709 Human
别名
- BRCA1/BRCA2 containing complex, subunit 5 antibody
- BRCC 5 antibody
- BRCC5 antibody
- DNA repair protein RAD51 homolog 1 antibody
- DNA repair protein rhp51 antibody
- FANCR antibody
- hRAD51 antibody
- HsRAD51 antibody
- HsT16930 antibody
- MRMV2 antibody
- Rad 51 antibody
- RAD51 antibody
- RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) antibody
- RAD51 homolog A antibody
- RAD51 homolog antibody
- RAD51 recombinase antibody
- RAD51, S. cerevisiae, homolog of antibody
- RAD51_HUMAN antibody
- RAD51A antibody
- RECA antibody
- RecA like protein antibody
- RecA, E. coli, homolog of antibody
- Recombination protein A antibody
see all

Western blot - Anti-Rad51 antibody [14B4] (ab213)

All lanes : Anti-Rad51 antibody [14B4] (ab213) at 1/500 dilution

Lane 1 : Jurkat whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : NCI-H929 whole cell lysate

Lysates/proteins at 30 µg per lane.

Secondary
All lanes : HRP-conjugated anti-mouse IgG antibody at 1/10000 dilution

Developed using the ECL technique.

Predicted band size: 37 kDa

10% SDS-PAGE

Running condition: 80V, 15min; 140V, 40 min

Transfer condition: Semi-dry, 18 V, 60 min (Nitrocellulose membrane)

Blocking condition: 5% non-fat milk in TBST, RT, 60 min.

Primary antibody incubation: 4°C overnight.

Washing condition: 5 ml TBST, 4 x 5 min.

Chemiluminescent HRP Substrate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Rad51 with ab213 at 1/100 dilution at 4°C overnight. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min (Cuisinart Electric Pressure Cooker #EPC-1200, choose "high pressure"). Endogenous peroxidase blocking: 3% H₂O₂, RT, 30minutes. Blocking: 1.5% goat serum (dilute goat serum by 1xPBS), RT, 30 min. Secondary antibody: ABC HRP Kit (Mouse IgG), 1:200, RT, 30min. Washing: PBS, 2 x 5 mins. Chromogen system: DAB.
Western blot - Anti-Rad51 antibody [14B4] (ab213)

All lanes : Anti-Rad51 antibody [14B4] (ab213) at 1/500 dilution

Lane 1 : Non-transfected 293T (-) whole cell extracts
Lane 2 : RAD51 shRNA transfected (+) 293T whole cell extracts

Lysates/proteins at 30 µg per lane.

Secondary
All lanes : HRP-conjugated anti-mouse IgG antibody

Developed using the ECL technique.

Predicted band size: 37 kDa

10% SDS-PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)

Immunohistochemical analysis of paraffin-embedded human esophagus tissue labeling Rad51 with ab213 at 1/100 dilution at 4°C overnight. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min (Cuisinart Electric Pressure Cooker #EPC-1200, choose "high pressure"). Endogenous peroxidase blocking: 3% H₂O₂, RT, 30minutes. Blocking: 1.5% goat serum (dilute goat serum by 1xPBS), RT, 30 min. Secondary antibody: ABC HRP Kit (Mouse IgG), 1:200, RT, 30min. Washing: PBS, 2 x 5 mins. Chromogen system: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)

Immunohistochemical analysis of paraffin-embedded BT483 xenoograft tissue labeling RAD51 with ab213 at 1/200 dilution. Nuclear staining is observed. Antigen Retrieval: EDTA based, pH 8.0 buffer, 15min.
Western blot - Anti-Rad51 antibody [14B4] (ab213)Image courtesy of an anonymous Abreview.

Anti-Rad51 antibody [14B4] (ab213) at 1/500 dilution + lysate prepared from murine NIH3T3 cells at 20 µg

Secondary
HRP conjugated anti-mouse IgG at 1/5000 dilution

Developed using the ECL technique.

Predicted band size: 37 kDa
Observed band size: 42,45 kDa
why is the actual band size different from the predicted?

Exposure time: 5 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)This image is courtesy of an anonymous Abreview

ab213 staining Rad51 in Human Lovo xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 20% serum for 20 minutes at room temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in TBST) for 1 hour. An undiluted HRP-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.
See Abreview

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

发表研究结果有使用 ab213？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab213 被引用在 69 文献中.

Salmina K et al. "Mitotic Slippage" and Extranuclear DNA in Cancer Chemoresistance: A Focus on Telomeres. Int J Mol Sci 21:N/A (2020). PubMed: 32316332
Horikoshi N et al. Pre-existing H4K16ac levels in euchromatin drive DNA repair by homologous recombination in S-phase. Commun Biol 2:253 (2019). PubMed: 31286070
Silva B et al. FANCM limits ALT activity by restricting telomeric replication stress induced by deregulated BLM and R-loops. Nat Commun 10:2253 (2019). PubMed: 31138795
Sahin ID et al. Crizotinib and PARP inhibitors act synergistically by triggering apoptosis in high-grade serous ovarian cancer. Oncotarget 10:6981-6996 (2019). PubMed: 31857852
Huang TH et al. The Histone Chaperones ASF1 and CAF-1 Promote MMS22L-TONSL-Mediated Rad51 Loading onto ssDNA during Homologous Recombination in Human Cells. Mol Cell 69:879-892.e5 (2018). PubMed: 29478807

View all Publications for this product

客户评价及客户问答

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Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Blocking step

Serum as blocking agent for 20 minute(s) · Concentration: 20% · Temperature: RT°C

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: pH9

Sample

Human Tissue sections (Lovo Xenograft)

Specification

Lovo Xenograft

Permeabilization

Fixative

Formaldehyde

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提交于 Jul 17 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

概述

产品名称

描述

宿主

特异性

Tested Applications & Species

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

骨髓瘤

同种型

轻链类型

研究领域

相关产品

Compatible Secondaries

Isotype control

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

组织特异性

疾病相关

序列相似性

结构域

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

文献 (69)

客户评价及客户问答

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Rad51 antibody [14B4]