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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Homologous Recomb.

Anti-Rad51抗体[14B4] (ab213)

  • Datasheet
  • SDS
Reviews (9)Q&A (11)References (69)

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Western blot - Anti-Rad51 antibody [14B4] (ab213)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)
  • Western blot - Anti-Rad51 antibody [14B4] (ab213)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)
  • Western blot - Anti-Rad51 antibody [14B4] (ab213)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)

Key features and details

  • Mouse monoclonal [14B4] to Rad51
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG2b

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概述

  • 产品名称

    Anti-Rad51抗体[14B4]
    参阅全部 Rad51 一抗
  • 描述

    小鼠单克隆抗体[14B4] to Rad51
  • 宿主

    Mouse
  • 特异性

    Specific for human Rad51.
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • 免疫原

    Recombinant full length protein corresponding to Human Rad51 aa 1-338.
    Database link: Q06609

  • 阳性对照

    • WB: RAD51 shRNA transfected (+) 293T whole cell extracts, Jurkat, Raji and NCI-H929 whole cell lysate. IHC-P: BT483 xenoograft tissue, human colon esophagus tissue.
  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 17th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.40
    Constituent: 100% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    单克隆
  • 克隆编号

    14B4
  • 骨髓瘤

    NS1
  • 同种型

    IgG2b
  • 轻链类型

    kappa
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Homologous Recomb.

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • Recombinant Protein

    • Recombinant human Rad51 protein (Active) (ab63808)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
IHC-P
Human
WB
Human
应用 Ab评论 说明
IHC-P (1)
1/100 - 1/1000.
WB (5)
1/500 - 1/3000. Predicted molecular weight: 37 kDa.

Please see WB protocol in image legend. 

说明
IHC-P
1/100 - 1/1000.
WB
1/500 - 1/3000. Predicted molecular weight: 37 kDa.

Please see WB protocol in image legend. 

  • 应用说明
    Is unsuitable for ICC/IF.
  • 靶标

    • 功能

      Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.
    • 组织特异性

      Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
    • 疾病相关

      Breast cancer
      Mirror movements 2
      Defects in RAD51 are found in a patient with microcephaly, mental retardation without bone marrow failure and pediatric cancers.
    • 序列相似性

      Belongs to the RecA family. RAD51 subfamily.
      Contains 1 HhH domain.
    • 结构域

      The nuclear localization may reside in the C-terminus (between 259 and 339 AA).
    • 翻译后修饰

      Ubiquitinated by the SCF(FBXO18) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA.
      Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.
    • 细胞定位

      Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
    • Target information above from: UniProt accession Q06609 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 5888 Human
      • Omim: 179617 Human
      • SwissProt: Q06609 Human
      • Unigene: 631709 Human
      • 别名

        • BRCA1/BRCA2 containing complex, subunit 5 antibody
        • BRCC 5 antibody
        • BRCC5 antibody
        • DNA repair protein RAD51 homolog 1 antibody
        • DNA repair protein rhp51 antibody
        • FANCR antibody
        • hRAD51 antibody
        • HsRAD51 antibody
        • HsT16930 antibody
        • MRMV2 antibody
        • Rad 51 antibody
        • RAD51 antibody
        • RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) antibody
        • RAD51 homolog A antibody
        • RAD51 homolog antibody
        • RAD51 recombinase antibody
        • RAD51, S. cerevisiae, homolog of antibody
        • RAD51_HUMAN antibody
        • RAD51A antibody
        • RECA antibody
        • RecA like protein antibody
        • RecA, E. coli, homolog of antibody
        • Recombination protein A antibody
        see all

      图片

      • Western blot - Anti-Rad51 antibody [14B4] (ab213)
        Western blot - Anti-Rad51 antibody [14B4] (ab213)
        All lanes : Anti-Rad51 antibody [14B4] (ab213) at 1/500 dilution

        Lane 1 : Jurkat whole cell lysate
        Lane 2 : Raji whole cell lysate
        Lane 3 : NCI-H929 whole cell lysate

        Lysates/proteins at 30 µg per lane.

        Secondary
        All lanes : HRP-conjugated anti-mouse IgG antibody at 1/10000 dilution

        Developed using the ECL technique.

        Predicted band size: 37 kDa



        10% SDS-PAGE

        Running condition: 80V, 15min; 140V, 40 min

        Transfer condition: Semi-dry, 18 V, 60 min (Nitrocellulose membrane)

        Blocking condition: 5% non-fat milk in TBST, RT, 60 min.

        Primary antibody incubation: 4°C overnight.

        Washing condition: 5 ml TBST, 4 x 5 min.

        Chemiluminescent HRP Substrate.

         

         

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)

        Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Rad51 with ab213 at 1/100 dilution at 4°C overnight. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min (Cuisinart Electric Pressure Cooker #EPC-1200, choose "high pressure"). Endogenous peroxidase blocking: 3% H2O2, RT, 30minutes. Blocking: 1.5% goat serum (dilute goat serum by 1xPBS), RT, 30 min. Secondary antibody: ABC HRP Kit (Mouse IgG), 1:200, RT, 30min. Washing: PBS, 2 x 5 mins. Chromogen system: DAB.

      • Western blot - Anti-Rad51 antibody [14B4] (ab213)
        Western blot - Anti-Rad51 antibody [14B4] (ab213)
        All lanes : Anti-Rad51 antibody [14B4] (ab213) at 1/500 dilution

        Lane 1 : Non-transfected 293T (-) whole cell extracts
        Lane 2 : RAD51 shRNA transfected (+) 293T whole cell extracts

        Lysates/proteins at 30 µg per lane.

        Secondary
        All lanes : HRP-conjugated anti-mouse IgG antibody

        Developed using the ECL technique.

        Predicted band size: 37 kDa



        10% SDS-PAGE

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)

        Immunohistochemical analysis of paraffin-embedded human esophagus tissue labeling Rad51 with ab213 at 1/100 dilution at 4°C overnight. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min (Cuisinart Electric Pressure Cooker #EPC-1200, choose "high pressure"). Endogenous peroxidase blocking: 3% H2O2, RT, 30minutes. Blocking: 1.5% goat serum (dilute goat serum by 1xPBS), RT, 30 min. Secondary antibody: ABC HRP Kit (Mouse IgG), 1:200, RT, 30min. Washing: PBS, 2 x 5 mins. Chromogen system: DAB.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)

        Immunohistochemical analysis of paraffin-embedded BT483 xenoograft tissue labeling RAD51 with ab213 at 1/200 dilution. Nuclear staining is observed. Antigen Retrieval: EDTA based, pH 8.0 buffer, 15min.

      • Western blot - Anti-Rad51 antibody [14B4] (ab213)
        Western blot - Anti-Rad51 antibody [14B4] (ab213)Image courtesy of an anonymous Abreview.
        Anti-Rad51 antibody [14B4] (ab213) at 1/500 dilution + lysate prepared from murine NIH3T3 cells at 20 µg

        Secondary
        HRP conjugated anti-mouse IgG at 1/5000 dilution

        Developed using the ECL technique.

        Predicted band size: 37 kDa
        Observed band size: 42,45 kDa
        why is the actual band size different from the predicted?


        Exposure time: 5 minutes
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)This image is courtesy of an anonymous Abreview

        ab213 staining Rad51 in Human Lovo xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 20% serum for 20 minutes at room temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in TBST) for 1 hour. An undiluted HRP-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.

        See Abreview

      实验方案

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet
      • SDS
    • 文献 (69)

      发表研究结果有使用 ab213?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab213 被引用在 69 文献中.

      • Salmina K  et al. "Mitotic Slippage" and Extranuclear DNA in Cancer Chemoresistance: A Focus on Telomeres. Int J Mol Sci 21:N/A (2020). PubMed: 32316332
      • Horikoshi N  et al. Pre-existing H4K16ac levels in euchromatin drive DNA repair by homologous recombination in S-phase. Commun Biol 2:253 (2019). PubMed: 31286070
      • Silva B  et al. FANCM limits ALT activity by restricting telomeric replication stress induced by deregulated BLM and R-loops. Nat Commun 10:2253 (2019). PubMed: 31138795
      • Sahin ID  et al. Crizotinib and PARP inhibitors act synergistically by triggering apoptosis in high-grade serous ovarian cancer. Oncotarget 10:6981-6996 (2019). PubMed: 31857852
      • Huang TH  et al. The Histone Chaperones ASF1 and CAF-1 Promote MMS22L-TONSL-Mediated Rad51 Loading onto ssDNA during Homologous Recombination in Human Cells. Mol Cell 69:879-892.e5 (2018). PubMed: 29478807
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Rad51 antibody [14B4]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      Serum as blocking agent for 20 minute(s) · Concentration: 20% · Temperature: RT°C
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: pH9
      Sample
      Human Tissue sections (Lovo Xenograft)
      Specification
      Lovo Xenograft
      Permeabilization
      No
      Fixative
      Formaldehyde
      Read More

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      提交于 Jul 17 2013

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