Application
ChIP
Sample
Mouse Tissue lysate - nuclear (Limb buds, forebrain, ESC)
Negative control
We perform ChIP-seq
Specification
Limb buds, forebrain, ESC
Detection step
Other
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 37% Formaldehyde (Sigma), dilu
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 37% Formaldehyde (Sigma), dilu
Positive control
We perform ChIP-seq
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/333
Additional data
Additional Notes
So let's talk about this god-forsaken Antibody.
It is published that it works. In good papers. In multiple papers. It even works in our hands - SOMETIMES. Once upon a time we ordered a Lot was fine and we got really nice ChIP-seq results out of it (GR214359-10), then other Lots don't work at all (GR221348-3, GR3194001-1 and GR3194001-2) . When we order several lots that don't work, for 400€ EACH TIME. No problem, ABCAM, take the money for this non-service. Let alone the time and material and cost on our part each time. Speaking of time. Non-working antibodies do delay projects and publications.
So for this particular antibody: If advertised as ChIP-grade - please provide a product that actually works! There should be someone in your team that can run a standard ChIP-seq before you send out a product for 400€ an order to many customers. Honest to god, I happily pay the price if the antibody actually works I'd even recommend it to other people. But like this I feel like searching for an alternative.
To other customers: If you want to do ChIP-seq or ChIP-qPCR experiments: Specifically ask NOT to get Lot# GR314001-1, GR314001-2 or GR221348-3!
Yours truly,
Daniel
Abcam response
This reviewer was sent free of charge replacement antibodies to try to optimize the assay. Please contact Scientific Support with any questions.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Daniel Ibrahim
Verified customer
提交于 Nov 12 2018