Anti-Rad21抗体(ab992)
Key features and details
- Rabbit polyclonal to Rad21
- Suitable for: ICC/IF, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Rad21抗体
参阅全部 Rad21 一抗 -
描述
兔多克隆抗体to Rad21 -
宿主
Rabbit -
特异性
The epitope recognized by Anti-Rad21 antibody (ab992) maps to a region between residue 575 and the C-terminus (residue 631) human Rad21 homolog using the numbering given in entry NP_006256.1 (GeneID 5885).
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经测试应用
适用于: ICC/IF, WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Gorilla, Chinese hamster, Orangutan, Elephant
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免疫原
Synthetic peptide corresponding to Human Rad21 (C terminal) conjugated to keyhole limpet haemocyanin. Represented a portion of human Rad21 encoded within exon 14 (LocusLink ID 5885).
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阳性对照
- WB: HeLa cell lysate, 293T, NIH3T3. IHC-P: Human breast fibroadenoma.
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常规说明
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
Our Abpromise guarantee covers the use of ab992 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. Used at a dilution of 1/200 for 30 min incubation (see Abreview for further information). |
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| WB | 1/500 - 1/5000. Predicted molecular weight: 72 kDa. Band observed at ~130 kDa. |
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| IP | Use a concentration of 1 - 4 µg/ml. |
靶标
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功能
Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway. -
序列相似性
Belongs to the rad21 family. -
结构域
The C-terminal part associates with the head of SMC1A, while the N-terminal part binds to the head of SMC3. -
翻译后修饰
Cleaved by separase/ESPL1 at the onset of anaphase. Cleaved by caspase-3 and caspase-7 at the beginning of apoptosis. The cleavage by ESPL1 and caspase-3 take place at different sites.
Phosphorylated; becomes hyperphosphorylated in M phase of cell cycle. The large dissociation of cohesin from chromosome arms during prophase may be partly due to its phosphorylation by PLK. -
细胞定位
Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, it is cleaved by separase/ESPL1, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. Once cleaved by caspase-3, the C-terminal 64 kDa cleavage product translocates to the cytoplasm, where it may trigger apoptosis. - Information by UniProt
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数据库链接
- Entrez Gene: 540966 Cow
- Entrez Gene: 5885 Human
- Entrez Gene: 19357 Mouse
- Entrez Gene: 314949 Rat
- Omim: 606462 Human
- SwissProt: Q3SWX9 Cow
- SwissProt: O60216 Human
- SwissProt: Q61550 Mouse
see all -
别名
- CDLS4 antibody
- Double-strand-break repair protein rad21 homolog antibody
- hHR21 antibody
see all
图片
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Western blot - Anti-Rad21 antibody (ab992)Anti-Rad21 antibody (ab992) at 1/1000 dilution + HeLa whole cell lysate
Developed using the ECL technique.
Predicted band size: 72 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa (possible cross reactivity) -
Western blot - Anti-Rad21 antibody (ab992)Anti-Rad21 antibody (ab992) at 1/2000 dilution + HeLa whole cell extract (extraction was achieved using RIPA buffer and 1% SDS) at 25 µg/ml
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/1500 dilution
Predicted band size: 72 kDa
A rad21 knock down negative control was employed.
This image is courtesy of an Abreview submitted on 9 September 2005. We do not have any further information relating to this image. -
Western blot - Anti-Rad21 antibody (ab992)All lanes : Anti-Rad21 antibody (ab992) at 2 µg/ml
Lane 1 : HeLa at 50 µg
Lane 2 : HeLa at 15 µg
Lane 3 : HeLa at 5 µg
Lane 4 : 293T at 50 µg
Lane 5 : NIH3T3 at 50 µg
Predicted band size: 72 kDa
Exposure time: 30 seconds
实验方案
文献
ab992 被引用在 124 文献中.
- Kentepozidou E et al. Clustered CTCF binding is an evolutionary mechanism to maintain topologically associating domains. Genome Biol 21:5 (2020). PubMed: 31910870
- Khoury A et al. Constitutively bound CTCF sites maintain 3D chromatin architecture and long-range epigenetically regulated domains. Nat Commun 11:54 (2020). PubMed: 31911579
- Jia Z et al. Tandem CTCF sites function as insulators to balance spatial chromatin contacts and topological enhancer-promoter selection. Genome Biol 21:75 (2020). PubMed: 32293525
- Lhoumaud P et al. NSD2 overexpression drives clustered chromatin and transcriptional changes in a subset of insulated domains. Nat Commun 10:4843 (2019). PubMed: 31649247
- Yamada T et al. Sensory experience remodels genome architecture in neural circuit to drive motor learning. Nature 569:708-713 (2019). PubMed: 31068695
