Anti-Rad17 (phospho S645)抗体(ab3620)
Key features and details
- Rabbit polyclonal to Rad17 (phospho S645)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Rad17 (phospho S645)抗体
参阅全部 Rad17 一抗 -
描述
兔多克隆抗体to Rad17 (phospho S645) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla, African green monkey, Orangutan -
免疫原
Synthetic phospho peptide (Human) - which represents a portion of human Rad 17around serine 645.
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常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antibodies that were not phospho-specific were removedby solid phase absorption. Antibodies specific for Rad 17pSer 645 were affinity purified using the phosphopeptideimmobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab3620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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WB | 1/500 - 1/5000. Detects a band of approximately 85-88 kDa (predicted molecular weight: 77 kDa). |
靶标
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功能
Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination. -
组织特异性
Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors). -
序列相似性
Belongs to the rad17/RAD24 family. -
翻译后修饰
Phosphorylated. Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or RFC4. -
细胞定位
Nucleus. Phosphorylated form redistributes to discrete nuclear foci upon DNA damage. - Information by UniProt
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数据库链接
- Entrez Gene: 5884 Human
- Omim: 603139 Human
- SwissProt: O75943 Human
- Unigene: 16184 Human
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别名
- CCYC antibody
- Cell cycle checkpoint protein antibody
- Cell cycle checkpoint protein RAD17 antibody
see all
图片
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Sample: Whole Cell Lysate (50
µ g/lane) from Hela cells treated with 10Gy IR or untreated.Rabbit anti-Rad 17 pSer645 (ab3620) used at 1:1,000 dilution
Sample: Whole Cell Lysate (50
µg/lane) from Hela cells treated with 10Gy IR or untreated. Rabbit anti-Rad 17 pSer645 (ab3620) used at 1:1,000 dilution
实验方案
文献 (4)
ab3620 被引用在 4 文献中.
- Alfano L et al. Depletion of the RNA binding protein HNRNPD impairs homologous recombination by inhibiting DNA-end resection and inducing R-loop accumulation. Nucleic Acids Res N/A:N/A (2019). PubMed: 30799487
- Shin MH et al. ATM-dependent phosphorylation of the checkpoint clamp regulates repair pathways and maintains genomic stability. Cell Cycle 11:1796-803 (2012). PubMed: 22453082
- Kumar A et al. Nuclear phosphoinositide 3-kinase beta controls double-strand break DNA repair. Proc Natl Acad Sci U S A 107:7491-6 (2010). WB, ICC/IF ; Mouse . PubMed: 20368419
- Jurvansuu J et al. Viral transport of DNA damage that mimics a stalled replication fork. J Virol 79:569-80 (2005). PubMed: 15596849